毕赤酵母表达系统优化策略概述  

Advance in the Research of Antimicrobial Peptides Gene Expression in Pichia pastor

作　　者：傅小蒙 孔令聪[1] 裴志花[1] 刘树明[1] 马红霞[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118

出　　处：《中国生物工程杂志》2015年第10期86-90,共5页China Biotechnology

基　　金：国家自然科学基金(31140026);教育部新世纪优秀人才项目(NCET-10-0174);吉林省教育厅"十一五"科学技术研究项目(2009第49号);吉林省世行贷款农产品质量安全项目(2011-Y05)资助项目

摘　　要：毕赤酵母(Pichia pastor)表达系统是近年发展起来的一种高效表达外源蛋白的系统,利用该系统表达外源基因具有良好的应用前景。尽管毕赤酵母表达系统具有比较完备的基因表达调控机制和对真核基因表达产物的加工修饰能力,但由于基因本身及表达系统等诸多因素,仍然存在外源蛋白表达产量很低甚至不表达的情况。针对毕赤酵母表达系统这一因素,对表达载体的优化,毕赤酵母菌株优化及发酵条件优化进行了综述,以期为外源基因在毕赤酵母中的高效表达提供理论基础。Pichia pastoris expression system is a kind of high efficiency expression system of exogenous protein developed in recent years, and it is promising to express exogenous gene in Pichia pastoris. Although the relatively complete mechanism of gene expression and regulation as well as the capability of performing eukaryotic post-translational modifications of Pichia pastoris expression system has been well known, some case with no expression or the low yield were still existed due to numerous factors of gene and expression system. The aim of the expression system of Pichia pastoris, the optimization of expression vectors, engineering strain and fermentation conditions were reviewed to lay the theory foundation that exogenous gene will be expressed efficiently in Pichia pastoris.

关 键 词：毕赤酵母 表达载体 菌株 发酵 优化 

分 类 号：Q786[生物学—分子生物学]