丹芪偏瘫胶囊挥发性成分的GC-MS指纹图谱研究  

Study on the GC-MS Fingerprint Spectrum of Volatile Components in Danqi Piantan Capsule

作　　者：宋洁瑾[1] 陈涛[1] 李进[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300193

出　　处：《中国药房》2015年第33期4716-4718,共3页China Pharmacy

基　　金：天津市西青区科技型中小企业发展专项资金项目(No.XQKC2013-022)

摘　　要：目的：建立丹芪偏瘫胶囊挥发性成分的气相色谱-质谱联用（GC-MS）指纹图谱。方法：采用GC-MS法。色谱条件：色谱柱为BPX 5,进样口温度为250℃,程序升温,载气为氦气,流速为1 ml/min,分流进样,分流比为50∶1,进样量为0.02μl;质谱条件：离子源为电子轰击,轰击能量为70 e V,离子源温度为200℃,传输线温度为250℃,扫描范围为40~500 amu,溶剂延迟2min。以色谱峰面积最大的色谱峰为参照峰,分析10批丹芪偏瘫胶囊的共有峰,采用《中药色谱指纹图谱相似度评价系统》进行相似度分析,采用美国国家标准与技术局（NIST）标准质谱数据库检索鉴定共有峰。结果：10批丹芪偏瘫胶囊有13个共有峰,相似度均〉0.92;经验证,10批丹芪偏瘫胶囊指纹图谱与对照指纹图谱具有较好的一致性。3号峰为当归独有,2、4、5、7、8、12号峰为石菖蒲独有。结论：所建立的指纹图谱专属性强、方法稳定,可为丹芪偏瘫胶囊质量评价提供依据。OBJECTIVE：To establish the GC-MS fingerprint spectrum of volatile components in Danqi piantan capsule. METHODS：GC-MS was performed. HPLC conditions：The column was BPX 5,temperature of injection was 250 ℃,respectively;carrier gas was He,flow rate was 1 ml/min,split injection,split ratio was 50∶1 and volume injection was 0.02 μl. MS conditions：ion source was EI,bombarding energy was 70 e V,temperature of ion source and transmission line was 200 ℃ and 250 ℃,respectively;scan range was 40-500 amu,solvent delay of 2 min. The peak with largest chromatographic peaks areas was reference peak,common peaks of 10 batches of Danqi piantan capsule were analyzed. Similarity evaluation system for chromatographic figerprint of TCM was adapted for the similarity analysis and NIST standard MS database was used to retrieve and identify the common peak.RESULTS：There were totally 13 common peaks in the 10 batches of Danqi piantan capsule with similarity degree〉0.92;according to the verification,the fingerprint spectrum was a dapted for the similarity analysis and reference fingerprint of 10 batches of Danqi piantan capsule showed good consistency. The No.3 peak was Angelica sinensis unique,the No.2,4,5,7,8,12 peak was Acorus tatarinowii unique. CONCLUSIONS：The established method is specific and stable,and can provide basis for the quality assessment for Danqi piantan capsule.

关 键 词：丹芪偏瘫胶囊 挥发性成分 指纹图谱 气相色谱-质谱联用 质量控制 

分 类 号：R927.1[医药卫生—药学]