四环素对费氏弧菌产生生毒物兴奋效应(Hormesis)的时间关系和机制  

Time dependence and mechanism of tetracycline Hormesis effects to Vibrio fischeri

作　　者：汤淼[1,2] 曾鸿鹄[1] 王大力[2] 王婷[2] 林志芬[2] 陈瑞[2] 莫凌云[1] 

机构地区：[1]桂林理工大学环境科学与工程学院,桂林541004 [2]污染控制与资源化研究国家重点实验室,同济大学环境科学与工程学院,上海200092

出　　处：《环境化学》2015年第11期1981-1987,共7页Environmental Chemistry

基　　金：同济大学污染控制与资源化研究国家重点实验室自主研究项目(PCRRY11003);国家自然科学基金(201177092,21377096);中央高校基本科研业务费专项资金(0400219181)资助

摘　　要：以费氏弧菌(Vibrio fischeri,V.fischeri)作为受试生物,盐酸四环素作为研究对象,测定了盐酸四环素对V.fischeri在0—24 h的发光强度和细菌生长OD600的作用,并初步探讨了四环素对V.fischeri的Hormesis效应的作用机制.研究表明,盐酸四环素对V.fischeri的发光强度表现出了时间依赖型的Hormesis效应,即Hormesis效应出现在细菌生长的延滞期及平台期;根据V.fischeri的发光机制并结合量子化学计算结果,提出了盐酸四环素通过提供质子促进发光反应,从而产生Hormesis效应的可能机制.The toxicity and mechanism of tetracycline hydrochloride on the luminescence and cell proliferation of Vibrio fischeri during 0—24 h were investigated in this paper. It was found that tetracycline exhibited time-dependent Hormesis effects on the bacterial luminescence because Hormesis effects were observed only in the adaptation and stationary periods. A possible mechanism of the Hormesis effects was then proposed based on the light emitting mechanisms and the quantum chemical parameters of tetracycline that was calculated by Gaussian 09. It was supposed that tetracycline at low doses act as proton donors to provide protons that are essential in the light emitting process,and thus stimulate the light emission of the bacteria.

关 键 词：毒物兴奋(Hormesis) 费氏弧菌(Vibrio fischeri) 四环素 时间效应 

分 类 号：X171.5[环境科学与工程—环境科学]