野生松茸子实体提取物生物活性的研究  

Study on the biologic activities of active components obtained from Tricholoma matsutake

作　　者：刘存芳[1] 

机构地区：[1]陕西理工学院化学与环境科学学院,汉中723000

出　　处：《药物分析杂志》2015年第11期1953-1957,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：陕西省科技厅社会发展科技攻关项目:中药的加工技术研究(2015SF270);汉中市科技发展专项计划项目(2013hzzx50)

摘　　要：目的:提取松茸中的脂溶性和水溶性物质,测试其抗菌、抗肿瘤和抗氧化活性,为其开发利用提供理论依据。方法:索氏法提取脂溶性物质,超声法提取水溶性物质,平板转种法测试抗菌活性,腹腔给药法测试抗肿瘤活性,H2O2/Fe2+法测试抗氧化活性。结果:松茸脂溶性物质有抗菌活性,抗肿瘤活性不显著;松茸水溶性物质有抗菌活性,抗肿瘤活性更为显著;松茸脂溶性物质和水溶性物质对·OH有清除作用。结论:松茸脂溶性和水溶性物质有抗菌、抗肿瘤和抗氧化活性,活性效果存在差异。Objective ：To extract oil-soluble components and water-soluble components from the powder of Tricholoma matsutake, and then investigate their antimicrobial activities, anti-tumor activities and anti-oxidation activities. Methods：The oil-soluble components were first extracted from powder of Tricholoma matsutake with aether recycling in Soxhelt, and then the water-soluble components were extracted by ultrasonic extraction in hot water. The antimicrobial activities ,anti-tumor activities and anti-oxidation activities were then investigated with the plate transfer method, intraperitoneal dosing method, and the method of n2 O2/Fe2 ＋ , respectively. Results： Oil- soluble components obtained from Tricholoma matsutake inhibited the growth of testing bacterium, but the anti-tumor activity was low. The antimicrobial activities of water-soluble components obtained from Tricholoma matsutake was feeble in inhibiting the growth of testing bacteria and sterilize them, and its anti-tumor activity was remarkably effective. Oil-soluble components and water-soluble components obtained from Tricholoma matsutake had obvious action to antioxidation activities of scavenging effect for · OH. Conclusion ： Oil- soluble components and watersoluble com- ponents from Tricholoma matsutake have antimicrobial activities ,anti-tumor activities and anti-oxidation activities, and there are differences among their activities.

关 键 词：野生真菌 松茸 脂溶性物质 水溶性物质 抗菌活性 抗肿瘤活性 抗氧化活性 

分 类 号：R917[医药卫生—药物分析学]