八角茴香总黄酮抗氧化活性研究  

Study on antioxidative effect of total flavonoids from Illicium verum

作　　者：王硕[1] 司建志 龚小妹[1] 周小雷[1] 缪剑华[1] 

机构地区：[1]广西药用植物研究所西南濒危药材资源开发国家工程实验室,广西南宁530023

出　　处：《食品工业科技》2015年第23期75-78,共4页Science and Technology of Food Industry

基　　金：国家科技支撑计划"八角饮片及提取物质量评价和生产过程标准研究"(2011BAI01B04)

摘　　要：本实验采用Fenton法测定了八角茴香总黄酮(FIVR)对羟基自由基(·OH)和超氧阴离子自由基(O_2^-·)的清除作用,并与维生素C(V_C)进行了比较,采用FRAP法测定了总抗氧化能力(T-AOC);采用腹腔注射D-半乳糖建立亚急性衰老小鼠模型。动物随机分为正常对照组、模型对照组、阳性对照组、FIVR高、中、低剂量组(200、100、50 mg/g·d)。实验48 d后处死动物,称量体重,计算器官指数,测定小鼠血清、肝脏和脑组织中的超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力。结果发现,八角茴香总黄酮(FIVR)对·OH、O_2^-·的清除率和T-AOC均随着浓度的增加而上升;模型组小鼠体重、肝脏、脑指数及血清、肝、脑组织中SOD和GSH-Px活性均明显低于正常组,建模成功;而FIVR各给药组及V_C组小鼠体重、肝脏、脑指数及血清、肝、脑组织中SOD和GSH-Px活力均高于模型组。说明八角茴香总黄酮(FIVR)具有明显的抗氧化活性。The clearance effect of total flavonoids from I llicium verum （FIVR）on ·OH, O2 ^-· were determined using Fenton method and compared with that of vitamine C.Total antioxidant capacity（T-AOC）were determined using FRAP method to evaluate FIVR in vitro antioxidant activity. The aging mice model was established by the intraperitoneal injection of D-galactose.The mice were randomly divided into normal control group,model group, positive control group, FiVR high, medium and low- dose groups （200, 100,50 mg/g. d）.After administrations for 48 d,the body weight of mice were determined,then the organ coefficients were counted.The content of SOD, GSH-Px of liver, brain tissue and in the blood was detected. From FIVR antioxidant experiment in vitro, the clearance rate of ·OH, O2 ^-· and T-AOC were increased with the increase of concentration.The body weight of mice,liver index, brain index and the activity of SOD, GSH-Px in liver tissue, brain tissue of model group were lower than normal group, which proved successful model;each FIVR dose group mice weight, liver index, brain index and the activity of SOD,GSH-Px in liver tissue and brain tissue were higher than model group.This study shows that FIVR has obvious antioxidation activity.

关 键 词：八角茴香总黄酮 抗氧化 超氧化物歧化酶 谷胱甘肽过氧化物酶 

分 类 号：TS201.2[轻工技术与工程—食品科学]