热激处理减轻黄瓜冷害与细胞壁代谢的关系  

Impact of heat shock treatment on cucumber cell wall composition and cell wall hydrolase activity

作　　者：沈丽雯 刘娟[1] 董红敏[1] 周心雅 佟慧玲 秦文[1] 

机构地区：[1]四川农业大学食品学院,四川雅安625014

出　　处：《食品工业科技》2015年第23期329-332,338,共5页Science and Technology of Food Industry

摘　　要：为了进一步阐明热激处理缓解黄瓜冷害症状的作用机理,研究热激处理对采后黄瓜果实冷害指数、细胞壁组分以及细胞壁降解酶活性的影响,将"金田208"黄瓜经42℃热水处理10 min后,于(2±1)℃下贮藏。贮藏期间定期测定黄瓜果实的冷害指数、果实硬度、细胞壁组分含量和细胞壁水解酶活性。实验结果表明:与对照组相比,热激处理可以显著(p<0.05)降低黄瓜果实受到低温胁迫时的冷害指数,能够降低果胶甲酯酶(PME)、多聚半乳糖醛酸酶(PG)、β-葡萄糖苷酶和纤维素酶的活性,能够延缓果胶和纤维素含量的下降。相关性分析结果表明,黄瓜的冷害与细胞壁组分(原果胶、可溶性果胶、纤维素)之间存在极显著(p<0.01)的相关性,与细胞壁水解酶PME和纤维素酶活性之间存在极显著(p<0.01)的相关关系,因此可以认为热激处理可以通过降低采后黄瓜果实细胞壁降解酶的活性而减少细胞壁组分的降解,从而维持细胞壁结构的完整性,使黄瓜果实表现出一定的抗冷性。In order to clarify the mechanism of how heat shock treatment alleviate the chilling injury of cucumber, the influence of hot shock treatment on the chilling injury index,cell wall compositions and cell wall degrading enzyme activity were studied, in a certain time interval, the chilling injury index, cell wall compositions and cell wall degrading enzyme activity were measured. ＂Jintian 208＂ cucumbers were put in 42 ℃ hot water for 10 min and then stored at（2 ±1 ）℃.The test results showed that heat shock treatment could significantly decrease（ p 〈 0.05） the chilling injury index by comparing with the control group, it also could reduce the activities of the pectinesterase （PME）, Polygalacturonase（PG） ,^-glucosidase and Cellulase, in turn, it could postpone the the reduction of the content of pectin and cellulose. The correlation analysis showed that there existed highly significant correlations between chilling injury indexs and cell wall components, cell wall hydrolase and cellulose activities （p 〈 0.01 ）. It could, therefore, be considered that the heat shock treatment can reduce the degradation of cell wall components by reducing the activity of cell wall degrading enzymes in cucumber fruits. In turn, the integrity of the cell wall structure can be maintained.Thus,the cucumber exhibits a certain cold resistance.

关 键 词：热激处理 黄瓜 冷害 细胞壁组分 细胞壁降解酶 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]