河豚鱼16S rRNA基因部分DNA序列分析及应用  

Partial DNA Sequence Analysis of 16S rRNA Gene in Puffer Fish

作　　者：陈文炳[1] 翁国柱 陈融斌[3] 缪婷玉[1] 邵碧英[1] 江树勋[1] 彭娟[1] 

机构地区：[1]福建出入境检验检疫局技术中心,福建福州350001 [2]莆田出入境检验检疫局,福建莆田351100 [3]集美大学水产学院,福建厦门361021

出　　处：《食品科学》2015年第21期140-144,共5页Food Science

基　　金：国家质量监督检验检疫总局科技计划项目(2013IK149)

摘　　要：应用16S rRNA基因聚合酶链式反应（polymerase chain reaction,PCR）扩增通用引物,对3属13种福建省搜集的河豚鱼样品与9个未知物种的河豚鱼样品的16S rRNA基因序列中的部分片段进行PCR扩增与脱氧核糖核酸（deoxyribonucleic acid,DNA）碱基序列测定,各物种序列长度在611~614 bp之间。应用DNAMAN V6软件进行样品间DNA序列同源性比对分析,建立样品间系统关系同源树。供试13个样品被划分为4个类群组,群间的同源率为87%,群内同源率为94%~100%。根据序列同源性分析结果,9个未知种名的样品被归类到3个类群组中,推测9个未知种名的样品为东方鲀属或腹刺鲀属。探讨了16S rRNA基因部分DNA序列测试及同源性分析技术在河豚鱼种属鉴别中应用的可能性。Partial fragments of the 16 S rRNA gene of puffer fish from 13 species belonging to 3 genus collected from Fujian province and 9 samples from unknown species were amplified by polymerase chain reaction（PCR） using the universal 16 S rRNA gene primers. The PCR products were sequenced, and the length of the obtained DNA fragments were 611–614 bp in all samples. The homology of DNA sequences among samples was analyzed with the DNAMAN V6 software, and the phylogenetic tree among samples was established. Thirteen samples were divided into 4 groups, with a homology of 87% among groups and 94%–100% between any two samples within the same group. Nine samples of unknown puffer fish species were classed into 3 groups which belonged to the Gastrophysus or Takifugu genus. The results obtained in this study can provide references for the application of 16 S rRNA gene sequencing and homological analysis to identify puffer fish at genus and species levels.

关 键 词：河豚鱼 聚合酶链式反应 16S RRNA基因 DNA测序 物种鉴定 

分 类 号：S917.4[农业科学—水产科学] Q78[生物学—分子生物学]