植物乳杆菌NCU116发酵胡萝卜浆对免疫抑制小鼠肠道黏膜免疫的影响  

Effect of Carrot Slurry Fermented with Lactobacillus plantarum NCU116 on Intestinal Mucosal Immunity in Immunosuppressed Mice

作　　者：谢俊华[1] 聂少平[1] 丁翘 余强[1] 胡婕伦[1] 熊涛[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品科学》2015年第21期201-206,共6页Food Science

基　　金：国家高技术研究发展计划(863计划)项目(2011AA100904)

摘　　要：目的:探讨植物乳杆菌NCU116发酵胡萝卜浆对免疫抑制小鼠肠道黏膜免疫的影响。方法:利用环磷酰胺建立免疫抑制小鼠模型,将小鼠随机分为5组,即正常对照组、模型组、胡萝卜原浆组、发酵胡萝卜浆组、灭活发酵胡萝卜浆组。小鼠以灌胃的方式给予胡萝卜原浆、发酵胡萝卜浆和灭活发酵胡萝卜浆后,分别测定各组小鼠胸腺指数和脾脏指数,酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)方法测定小肠组织白细胞介素-2(interleukin-2,IL-2)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、IL-10和分泌型免疫球蛋白A(secretory immunglobulin A,sIgA)含量,小肠组织病理学观察及直接免疫荧光检测IgA分泌B细胞数。结果:实验组与模型组相比,植物乳杆菌NCU116发酵胡萝卜浆能显著提高小鼠胸腺和脾脏指数,增加Ig A分泌B细胞数量,提高细胞因子IL-2和sIgA的水平,改善肠道形态结构,但TNF-α和IL-10含量没有显著增加。结论:植物乳杆菌NCU116发酵胡萝卜浆能有效拮抗环磷酰胺对小鼠免疫功能的抑制作用和肠道结构损伤作用,提高免疫抑制小鼠的免疫功能和改善肠道形态结构。Objective： To study the effect of carrot slurry fermented with Lactobacillus plantarum NCU116 on intestinal mucosal immunity in immunosuppressed mice. Methods： Immunosuppressed mouse model was established by intraperitoneal injection of cyclophosphamid. Forty mice were randomly divided into 5 groups： blank control group, model control group, carrot slurry group, fermented carrot slurry group and sterilized fermented carrot slurry group. The mice were administered with slurry fermented with Lactobacillus plantarum NCU116 and sterilized fermented carrot slurry at a dose of 10 m L/（kg·d） for 21 consecutive days. At 24 h after the last administration, the mice in each group were sacrificed. Thymus and spleen indices were determined, the contents of IL-2, TNF-α, IL-10 and sIgA in small intestine were determined by using quantitative sandwich enzyme-linked immunosorbent assay（ELISA） kits, small intestine morphology was observed by hematoxylin-eosin（HE） stained section and immunofluorescence was used to assay IgA-secreting B cells. Results： Compared to the model control, carrot slurry fermented with Lactobacillus plantarum NCU116 could significantly increase thymus and spleen indices and the number of Ig A-secreting B cells, enhance IL-2 and sIgA levels and improve the intestinal morphological structure. But the concentrations of TNF-α and IL-10 were not significantly increased. Conclusion： Carrot slurry fermented with Lactobacillus plantarum NCU116 can effectively antagonize cyclophosphamide-induced immune suppression and ameliorate intestinal morphological damage in mice, enhance immune function and improve intestinal morphology in immunosuppressed mice.

关 键 词：植物乳杆菌NCU116 发酵胡萝卜浆 肠道黏膜免疫 

分 类 号：TS201.3[轻工技术与工程—食品科学]