分散液液微萃取-高效液相色谱法测定枸杞中α-生育酚  

Determination of α-Tocopherol in Goji Berries(Lycium barbarum) Using Dispersive Liquid-Liquid Microextraction Combined with High Performance Liquid Chromatography

作　　者：曹江平[1] 刘世巍[1] 解启龙[2] 易宗慧[1] 周继梅[1] 张玉龙[1] 

机构地区：[1]宁夏师范学院化学化工学院,宁夏固原756000 [2]中国科学院山西煤炭化学研究所煤转化国家重点实验室,山西太原030001

出　　处：《食品科学》2015年第22期122-125,共4页Food Science

基　　金：宁夏自然科学基金资助项目(NZ14275;NZ13216);宁夏师范学院科研项目(YB201433)

摘　　要：建立基于分散液液微萃取样品前处理法,并结合高效液相色谱紫外检测技术测定枸杞中α-生育酚的含量,考察影响分散液液微萃取的因素包括萃取剂和分散剂类型及用量、p H值、离子强度、萃取时间和萃取温度。方法的线性范围为100~50 000μg/L,r=0.999 7。在最优条件下,日内和日间重复性分别为2.9%和6.5%,检出限为3.1μg/L,定量限为10.2μg/L。并将该法成功应用于3种市售枸杞中α-生育酚含量的测定,加标回收率为85.7%~106.3%,相对标准偏差为0.68%~4.62%。方法具有有机溶剂用量少、操作简单快捷、准确度和灵敏度高、重复性好等优点。A novel method for the determination of α-tocopherol in Goji berries（Lycium barbarum） was developed using dispersive liquid-liquid microextraction（DLLME） coupled with high performance liquid chromatography by UV detection（HPLC-UV）.The influence of DLLME experimental parameters including type and volume of extraction solvents and dispersers,sample solution pH,ionic strength,extraction time and temperature on extraction efficiency were examined.The method offered excellent linearity over a range of 100-50 000 μg/L with correlation coefficient（r） of 0.999 7.Under the optimum conditions,intra-day and inter-day repeatability values,expressed as relative standard deviation（RSD）,were2.9%and 6.5%,respectively.The limit of detection（LOD） and limit of quantitation（LOQ） were 3.1 μg/L and 10.2 μg/L,respectively.The developed method was successfully applied to the determination of α-tocopherol in 3 types of commercial Goji berry products.Average recoveries varied from 85.7%to 106.3%,with RSD in the range of 0.68%-4.62%.In addition,the DLLME technique had the advantages of simplicity,rapidity and low organic solvent consumption,excellent precision,high sensitivity and good repeatability.

关 键 词：分散液液微萃取 枸杞 Α-生育酚 

分 类 号：TS207.3[轻工技术与工程—食品科学]