外源Ca2＋及钙离子信号抑制剂对茶树抗寒性的影响  

Effects of Exogenous Calcium and Inhibitors of Calcium Signaling Transduction Pathway on Cold Resistance of Tea Plant

作　　者：黄玉婷[1] 钱文俊[1,2] 王博[1] 曹红利[1] 王璐[1] 郝心愿[1] 王新超[1] 杨亚军[1] 

机构地区：[1]中国农业科学院茶叶研究所、国家茶树改良中心农业部茶树生物学与资源利用重点实验室,浙江杭州310008 [2]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《茶叶科学》2015年第6期520-526,共7页Journal of Tea Science

基　　金：国家自然科学基金(31170650);国家茶叶产业技术体系(CARS-23);浙江省农业新品种选育重大专项(2012C2905-3)

摘　　要：钙离子信号通路在植物抗寒响应中起着重要作用。为了解钙离子信号通路与茶树抗寒性之间的关系,采用人工气候室模拟低温胁迫和外源施用钙调素(CaM)抑制剂W-7[N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide]、钙信号通道抑制剂LaCl_3及CaCl_2溶液的方法,测定了低温胁迫下茶树叶片各种与抗寒相关的生理指标的变化情况。检测结果表明,钙离子信号抑制剂W-7、LaCl_3处理均能提高茶树叶片相对电导率,提高茶树叶片中丙二醛(MDA)、超氧阴离子自由基(O_2·^-)、脯氨酸的含量,抑制超氧化物歧化酶(SOD)的活性;而CaCl_2溶液处理茶树叶片相对电导率及叶片中MDA、超氧阴离子自由基和脯氨酸的含量降低,SOD活性提高。表明钙离子信号系统在茶树抗寒过程中发挥了重要作用。Thecalciumsignal transduction pathway plays a pivotal role in the response to the stress of low temperatures. In order toverify the relationship between calciumsignal transduction pathwayand cold resistance of tea plant,the changes of some cold resistance related indexwere measured underartificiallow temperature stress in climatron accompanied with using exogenous CaCl2,CaM inhibitor W-7[N-(6-Aminohexyl)-5-chloro-1-naphthalene-sulfonamide]andcalcium signal channel inhibitorLaCl3, respectively.Calcium signaling transduction pathway inhibitors W-7and La Cl3improved electrolyte leakage,enhanced contents ofmalondialdehyde(MDA),superoxide anion radical(O2·-) and proline,reduced the activities of superoxide dismutase (SOD)compared with control treatment, however,CaCl2treatmentshowed opposite effects.Itshowed thatcalciumsignaling system play an important role in theresponse tolow temperature stressintea plant.

关 键 词：茶树 抗寒 钙离子信号通路抑制剂 CA2+ 

分 类 号：S571.1[农业科学—茶叶生产加工] O614.231[农业科学—作物学]