1型糖尿病大鼠模型早期胃平滑肌功能的改变研究  

Alterations in the functions of gastric smooth muscle in type 1 diabetic rats

作　　者：郝璋森[1,2] 梁红玉[1] 侯聪聪[1] 佟苗苗[1] 刘帅[1] 蒋建东[3] 赵丁[1,3,4] 

机构地区：[1]河北医科大学药学院,石家庄050017 [2]石家庄市第四医院药剂科,石家庄050000 [3]中国医学科学院/北京协和医学院药物研究所,天然药物活性物质与功能国家重点实验室,北京100050 [4]河北医科大学中西医结合研究所,石家庄050017

出　　处：《中国比较医学杂志》2015年第11期52-58,共7页Chinese Journal of Comparative Medicine

基　　金：河北省自然科学基金石药基金项目(C2011206175);河北省科技厅科技支撑计划项目(142777114D)

摘　　要：目的探讨1型糖尿病(type 1 diabetes mellitus)早期大鼠离体胃平滑肌收缩、舒张反应的改变。方法雄性SD大鼠,腹腔注射65 mg/kg链脲佐菌素(STZ)溶液诱导1型糖尿病大鼠模型。采用离体器官肌张力分析方法,以电场刺激及激动剂诱发胃各部位平滑肌收缩、舒张反应,观察糖尿病早期大鼠离体胃平滑肌功能的改变。结果 (1)电场刺激诱发1型糖尿病大鼠胃底平滑肌收缩反应小于正常大鼠(P<0.05),且收缩幅度紊乱,参差不齐;(2)正常大鼠及1型糖尿病大鼠胃体平滑肌收缩反应、胃窦自发收缩活动均无明显改变;(3)与正常大鼠相比,电场刺激诱发1型糖尿病大鼠胃底平滑肌舒张反应明显增加,而幽门环形肌收缩反应明显增加(P<0.01)。结论 STZ造模4周的1型糖尿病大鼠胃平滑肌收缩舒张功能的变化中,胃各部位敏感性不同;胃底平滑肌收缩反应明显减小且紊乱,舒张反应明显增强;幽门环形肌舒张反应明显减小。提示在1型糖尿病早期大鼠胃平滑肌功能改变中,胃底和幽门可能最先发生病变,且主要源自神经调节的改变。Objective To investigate the alteration of contractile and relaxant responses of the isolated gastric smooth muscle in early type 1 diabetic rats. Methods Male SD rats were administrated 65 mg / kg sterptozocin（ STZ） by intraperitoneal injection. Using the method of organ bath,contractile and relaxant responses were induced by electric field stimulation（ EFS） and agonists on gastric smooth muscle in rats,we observe the alterations of contractile and relaxant responses of the gastric smooth muscle in early type 1 diabetic rats. Results（ 1） In type 1 diabetic rats,the contractile responses were decreased,and the contractile amplitudes were disordered significantly,compared with that of in normal rats（ P〈0. 05）;（ 2） There were no differences in the EFS-induced contractions on gastric body smooth muscle and thespontaneous contractions on gastric antrum in normal rats and type 1 diabetic rats;（ 3） Compared with normal rats,both the relaxations on gastric fundus smooth muscle,and the contractions on pylorus circular muscle were increased in type 1diabetic rats（ P〈0. 01）. Conclusions Based on the changes of contractile and relaxant responses on gastric smooth muscle in STZ-induced type 1 diabetic 4 weeks rats,the damages of gastric fundus and pylorus seem to occur first,and the alterations of neuromodulation are main reason,which affects the contractile and relaxant functions of gastric smooth muscle in early type 1 diabetic rats.

关 键 词：糖尿病 胃平滑肌 电场刺激 神经损伤 大鼠 

分 类 号：R-332[医药卫生]