PTEN基因沉默对骨髓间充质干细胞生物学特性的影响  

PTEN silencing effects on biological properties of bone marrow mesenchymal stem cells

作　　者：陈毅[1] 陆晓东[1] 刘丹平[2] 屠冠军[3] 

机构地区：[1]浙江大学金华医院,浙江省金华市321000 [2]辽宁医学院附属第一医院,辽宁省锦州市121001 [3]中国医科大学附属第一医院,辽宁省沈阳市110001

出　　处：《中国组织工程研究》2015年第41期6590-6594,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81070971)~~

摘　　要：背景:脊髓损伤后脊髓自身的修复和再生能力有限,细胞移植治疗脊髓损伤具有很强的临床应用价值和前景。目的:探讨PTEN基因沉默对骨髓间充质干细胞生物学特性的影响,以期为组织工程提供更优良的种子细胞。方法:采用脂质体法转染PTEN基因特异的si RNA沉默PTEN基因,RT-PCR检测PTEN基因的表达,通过细胞生长曲线、细胞周期、Transwell实验观察PTEN基因修饰后细胞生物学特性的变化。结果与结论:PTEN基因在骨髓间充质干细胞中高表达并且siR NA成功干扰了PTEN基因的表达,PTEN基因沉默后的细胞具有更强的存活、增殖、迁移能力,更适合作为组织工程的种子细胞。BACKGROUND： The self-renew and regeneration capacity of the injured spinal cord is thought to be limited. Accordingly, cell transplantation is one potential strategy for promoting functional recovery after spinal cord injury. OBJECTIVE： To explore the effects of PTEN silencing on the biological properties of bone marrow mesenchymal stem cells, hoping to offer better seed cells for tissue engineering. METHODS： Bone marrow mesenchymal stem cells were transfected with specific siR NA-silenced PTEN gene using the liposome method, and then RT-PCR was used to detect the mR NA expression of PTEN. Variation of biological properties of PTEN-transfected cells were detected by the way of MTT assay, cell cycle analysis, and Transwell assay. RESULTS AND CONCLUSION： PTEN is expressed highly in bone marrow mesenchymal stem cells, which is successfully interfered by si RNA. PTEN-silenced cells have stronger survival, proliferation and migration abilities, which become a kind of better seed cells for tissue engineering.

关 键 词：骨髓 间质干细胞 PTEN磷酸水解酶 RNA干扰 组织工程 干细胞 骨髓干细胞 骨髓间充质干细胞 PTEN基因 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]