隔药灸“天枢”、“气海”穴对慢性炎性内脏痛大鼠血清P物质、TNF-α及PGE2的影响  

Effect of Herbal-partitioned Moxibustion at Tianshu(ST25) and Qihai(CV6) on Pain-related Behaviors and Emotion in Rats with Chronic Inflammatory Visceral Pain

作　　者：黄燕[1] 李志元[1] 杨延婷[1] 赵嬿[1] 张丹[2] 吴焕淦[2] 施征[2] 张翠红[2] 朱毅[2] 马晓芃[2] 

机构地区：[1]上海中医药大学,上海201203 [2]上海市针灸经络研究所,上海200030

出　　处：《中华中医药学刊》2015年第12期2836-2840,I0021,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81273843);国家自然科学基金青年科学基金项目(81202754);国家重点基础研究发展计划(973计划)项目(2015CB554500);上海市卫生和计划生育委员会项目(20144Y0153)

摘　　要：目的:观察隔药灸天枢、气海穴对慢性炎性内脏痛大鼠痛行为和血清P物质、TNF-α及PGE2的影响,探讨艾灸对慢性炎性内脏痛镇痛效应的外周机制。方法:32只SD大鼠随机分为正常组、模型组、隔药灸组和假灸组。除正常组外,其余三组均采用三硝基苯磺酸灌肠制备慢性炎性内脏痛大鼠模型。隔药灸组采用隔药灸天枢、气海穴治疗;假灸组仅在穴位上放置药饼和艾炷,不点燃艾炷;模型组和正常组均不进行治疗,只做与隔药灸组相同的固定。治疗结束后,检测大鼠腹壁撤回反射(abdominal withdrawal reflex,AWR)评分、机械性缩足反射阈值(mechanical withdrawal threshold,MWT)和热缩足潜伏期(thermal withdrawal latency,TWL);采用ELISA法检测血清P物质、TNF-α和PGE2的表达水平。结果:与正常组比较,模型组大鼠AWR评分在各个扩张压力下均显著升高(P<0.01),MWT、TWL均显著降低(P<0.05,P<0.01);与模型组和假灸组比较,隔药灸组大鼠AWR评分在各个扩张压力下均降低(P<0.05),MWT、TWL均明显升高(P<0.05,P<0.01)。与正常组比较,模型组大鼠血清P物质、TNF-α及PGE2均明显升高(P<0.01);与模型组和假灸组比较,隔药灸组大鼠血清P物质、TNF-α及PGE2均显著降低(P<0.01)。结论:隔药灸对慢性炎性内脏痛大鼠有镇痛作用;隔药灸能有效降低慢性炎性内脏痛大鼠血清P物质、TNF-α、PGE2水平进而发挥镇痛效应。Objective： To observe the effect of herbal-partitioned moxibustion（ HPM） on pain-related behavior and SP,TNF-α and PGE2 in serum in a rat model of chronic inflammatory visceral pain and to investigate the peripheral mechanism of this effect. Methods： Thirty-two Sprague-Dawley（ SD） male rats were randomly divided into four groups： normal group,model group,HPM group and sham group. Except for the normal group,rats in the other three groups were clystered with mixed liquor of trinitrobenzene sulfonic acid（ TNBS） and 50% ethanol to induce the chronic inflammatory visceral pain model. Rats in the HPM group were treated with HPM at bilateral Tianshu（ ST25） and Qihai（ CV6）. Rats in the sham group were only laid with herb cakes and moxa cones on the same acupoints as HPM group but without lighting the moxa cones. Rats in the normal group and the model group were only fixed as those in the HPM group without treatment. After treatment,abdominal withdrawal reflex（ AWR） score,mechanical withdrawal threshold（ MWT）and thermal withdrawal latency（ TWL） were adopted to detect the visceral and somatic pain. ELISA was employed to test the levels of SP,TNF-α and PGE2 in serum. Results： Compared with the normal group,AWR scores of the model group were significantly increased under different stimulus expansion pressure levels（ P〈 0. 01）. MWT and TWL were significantly decreased（ P 〈0. 05,P〈 0. 01）. Compared with the model group and the sham group,the AWR score of the HPM group was decreased significantly（ P〈 0. 05）. MWT and TWL were significantly increased（ P 〈0. 05,P〈 0. 01）. Compared with the normal group,the levels of SP,TNF-α and PGE2 expressed in serum of the model group were significantlyincreased（ P 〈0. 01）. Compared with the model group and the sham group,the levels of SP,TNF-α and PGE2 in serum of the HPM group was decreased significantly（ P 〈0. 01）. Conclusion： HPM has analgesic effect on chronic inflammatory visceral pain. It can markedly

关 键 词：灸法 慢性内脏痛 炎症性肠病 P物质 TNF-Α PGE2 

分 类 号：R-332[医药卫生]