JAK1/STAT1在EGFR 19-Del之NSCLC中的表达及其相关性研究  

The study on expression of JAK1/STAT1 and its correlation in NSCLC with EGFR exon 19 deletion

作　　者：杨锋[1] 王磊[1] 钟代星[1] 杨三虎[1] 

机构地区：[1]第四军医大学唐都医院胸腔外科,西安710038

出　　处：《临床与病理杂志》2015年第11期1967-1973,共7页Journal of Clinical and Pathological Research

摘　　要：目的:检测信号分子p-JAK1、p-STAT1在EGFR外显子19缺失突变(以下简称EGFR 19-Del)的非小细胞肺癌(non-small-cell lung cancer,NSCLC)组织样本中的表达,探讨EGFR突变后下游信号通路JAK/STAT表达之间的相关性,为进一步研究EGFR 19-Del的NSCLC发生发展机制提供依据。方法:收集经实时荧光定量PCR——ARMS法筛选的125例EGFR 19-Del的NSCLC组织,应用免疫组织化学法检测该组织样本中p-JAK1和p-STAT1的表达,统计分析二者在不同临床病理特点中的表达差异以及表达相关性。结果:p-JAK1、p-STAT1阳性表达主要定位于细胞质和细胞核中。在125例EGFR 19-Del的NSCLC组织样本中,p-JAK1、p-STAT1阳性率分别为83.2%、100%。p-JAK1、p-STAT1在鳞癌、腺癌两组间的表达无统计学差异(P=0.440;P=0.822);p-JAK1、p-STAT1在总的NSCLC、腺癌的不同病理分级、TNM分期组间差异显著(P值均<0.01),而二者在鳞癌的不同病理分级、TNM分期组间无统计学差异(P值均>0.05)。p-JAK1与p-STAT1在总的NSCLC、腺癌、鳞癌中的表达率和表达水平存在相关性(P值均<0.05,rs值均>0.428);二者在病理分级的高、中分化组中有很好的相关性(P值均<0.01,rs值均>0.525),而在低分化组中的相关性不大(P>0.05);二者在TNM分期组中的早、晚期均有很好的相关性(rs=0.567,P=0.000;rs=0.477,P=0.000)。结论:p-JAK1、p-STAT1在EGFR 19-Del NSCLC组织样本中有很高的表达率,且p-JAK1、p-STAT1在病理分类、病理分级及TNM分期组间的表达有很好的相关性,说明JAK1/STAT1信号通路很可能与EGFR 19-Del的NSCLC发生发展有关,为进一步研究EGFR 19-Del之NSCLC发生发展机制提供重要依据。Objective： The expression of p-JAK1, p-STAT1 was investigate and the correlation of p-JAK1 expression with p-STAT1 was analyzed in NSCLC with EGFR 19-Del. Methods： A total of 125 NSCLC samples with EGFR 19-Del were selected by ARMS, p-JAK1 and p-STAT1 were detected in those samples using immunochemistry and the correlation of their expression were statistically analyzed. Results： The expression of p-JAK1, p-STAT1was mainly located in cytoplasm and nucleus. In those cases, the expression rates of p-JAK1, p-STAT1 protein were 83.2% and 100%. There were no significant differences of p-JAK1 and p-STAT1 expression level between the squamous carcinoma group and the adenocarcinoma group （P=0.440; P=0.822）. There were significant differences of p-JAK1, p-STAT1 among the different pathological grades and TNM stages in the NSCLC and the adenocarcinoma （P〈0.01）, while there was no statistical difference in squamous carcinoma （P〉0.05）. The correlation of p-JAK1 with p-STAT1 for the expression rate and the level were obvious （P〈0.05, r＋〉0.428） in the NSCLC, adenocarcinoma and squamous cell carcinomas; The correlations ofp-JAK1 with p-STAT1 expression were also obvious in well and middle differentiation groups （P〈0.01, r＋〉0.525）, but little correlation in low differentiation group ofNSCLC （P〉0.05）; Also there were good correlations in the TNM staging I - II, III-1V groups in NSCLC. Conclusion： The p-JAK1, p-STAT1 protein were highly expressed in NSCLC with EGFR 19-Del, and there were good correlations of p-JAK1 expression with p-STAT1 in the pathological classification, pathologic stages and TNM stages, which suggest that JAK1/STAT 1 signal pathway may be associated with the development of NSCLC, all that would provide important basis for the further study on the mechanism of NSCLC with EGFR 19 deletion mutation.

关 键 词：JAK1 STAT1 非小细胞肺癌 EGFR 19缺失 

分 类 号：R734.2[医药卫生—肿瘤]