Expression Pattern of phb2 and Its Potential Function in Spermatogenesis of Scallop(Chlamys farreri)  

作　　者：HAN Tiantian MA Xiaoshi LIANG Shaoshuai GAO Beibei ZHANG Zhifeng 

机构地区：[1]Ministry of Education Key Laboratory of Marine Genetics and Breeding,College of Marine Life Sciences,Ocean University of China

出　　处：《Journal of Ocean University of China》2015年第6期1034-1040,共7页中国海洋大学学报（英文版）

基　　金：supported by the National High Technology Research and Development Program of China(863 Program)(2012AA10A402);the Natural Science Foundation of Qingdao(11-2-4-1(10)-jch)

摘　　要：Prohibitin(PHB) participates in several biological processes including apoptosis, transcription regulation and suppression of cell proliferation in mammals. In this study, we cloned the full-length c DNA of prohibitin 2(Cf-phb2) from the testis of scallop(Chlamys farreri). The deduced amino acid sequence presented a characteristic of PHB family with the PHB domain, and clustered with PHB2 of other species. Temporal and spatial expression of Cf-phb2 in testis during the reproductive cycle was detected by quantitative real-time PCR(q RT-PCR) and in situ hybridization. The expression of Cf-phb2 in the testis increased when testis developed from the resting stage to mature stage. The m RNA abundance of Cf-phb2 was the highest at mature stage, which was about 15-fold higher than that at proliferative stage. The expression of Cf-phb2 could be detected by in situ hybridization in all types of germ cells in testis, including spermatogonia, spermatocytes, spermatids and spermatozoa. The intensity of the signal increased with the spermatogenesis and was the highest in spermatids, which suggested that CF-PHB2 might affect the spermatogenesis of C. farreri.Prohibitin (PHB) participates in several biological processes including apoptosis, transcription regulation and suppression of cell proliferation in mammals. In this study, we cloned the full-length cDNA of prohibitin 2 (Cf-phb2) from the testis of scallop (Chlamys farreri). The deduced amino acid sequence presented a characteristic of PHB family with the PHB domain, and clustered with PHB2 of other species. Temporal and spatial expression of Cf-phb2 in testis during the reproductive cycle was detected by quantitative real-time PCR (qRT-PCR) and in situ hybridization. The expression of Cf-phb2 in the testis increased when testis developed from the resting stage to mature stage. The mRNA abundance of Cf-phb2 was the highest at mature stage, which was about 15-fold higher than that at proliferative stage. The expression of Cf-phb2 could be detected by in situ hybridization in all types of germ cells in testis, including spermatogonia, spermatocytes, spermatids and spermatozoa. The intensity of the signal increased with the spermatogenesis and was the highest in spermatids, which suggested that CF-PHB2 might affect the spermatogenesis of C. farreri.

关 键 词：phb2 SPERMATOGENESIS TESTIS CHLAMYS FARRERI 

分 类 号：S917.4[农业科学—水产科学]