应用iTRAQ对唐氏综合征胎儿脐带血的蛋白质组学研究  

Proteomics study of cord blood in Down′s syndrome fetuses by iTRAQ

作　　者：文锦丽[1] 林秀华[1] 郭辉[1] 李武县[1] 林琳华[2] 戴勇[1] 

机构地区：[1]暨南大学第二临床学院/深圳市人民医院临床医学研究中心,广东深圳518020 [2]暨南大学第二临床学院/深圳市人民医院产前诊断中心,广东深圳518020

出　　处：《检验医学与临床》2015年第23期3461-3462,3465,共3页Laboratory Medicine and Clinic

基　　金：广东省深圳市科技计划资助项目(201202121);广东省深圳市科创委重点资助项目(CXZZ20130321090846345)

摘　　要：目的筛选并鉴定唐氏综合征胎儿与健康对照者差异性表达蛋白质,以寻找唐氏综合征胎儿的生物标记物。方法应用同位素标记相对和绝对蛋白定量法和功能聚类分析法筛查和分析唐氏综合征胎儿脐带血和正常胎儿脐带血的差异表达蛋白。结果共筛选鉴定到506种差异表达蛋白,其中最显著的3种蛋白分别是基质蛋白-3、胸腺肽β10和骨桥蛋白。结论唐氏综合征胎儿和正常胎儿脐带血血清蛋白存在较多差异表达蛋白,其中基质蛋白-3、胸腺肽β10和骨桥蛋白等显著差异蛋白可能作为潜在的临床筛查生物标志物。Objective To identify the differentially expressed proteins between fetuses with Down′s syndrome and healthy control,so as to screen the potential biomarkers of fetuses with Down′s syndrome.Methods To investigate the differentially expressed proteins in cord blood of fetus with Down′s syndrome and normal fetus using isobaric tagging for relative and absolute protein quantification and gene gene ontology analysis.Results A total of 506 differentiate proteins were identified,in which 3proteins（Matrin-3,Thymosin beta 10 and Osteopontin）were significant.Conclusion The proteomics of the serum are significant different among normal fetus and fetus with Down′s syndrome.Some of the protein,such as Matrin-3,Thymosin beta 10 and Osteopontin,may have potential applying value in clinic screening pratice.

关 键 词：唐氏综合征 蛋白质组学 同位素标记相对和绝对蛋白定量法 功能聚类分析法 

分 类 号：R714.5[医药卫生—妇产科学]