采用石墨烯修饰的电化学免疫传感器检测猪肉中的己二烯雌酚含量  

Preparation of an electrochemical immunosensor modified with graphene for detection of dienestrol in pork

作　　者：张洁[1] 吴珺[2] 王传现[3] 邵科峰[2] 陈昌云[2] 赵波[2] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]南京师范大学化学与材料科学学院,南京210097 [3]上海出入境检验检疫局,上海200135

出　　处：《中国药科大学学报》2015年第6期683-688,共6页Journal of China Pharmaceutical University

基　　金：江苏省科技厅科技支撑计划资助项目(No.BE2014720);江苏省农业科技自主创新资金资助项目(No.CX(14)2127)~~

摘　　要：利用石墨烯及己二烯雌酚修饰电极,研制了一种用于检测己二烯雌酚的高灵敏新型电化学免疫传感器;通过竞争结合,以铁氰化钾为探针,实现了对己二烯雌酚的高灵敏快速检测。结果表明：研制的石墨烯/己二烯雌酚电化学免疫传感器具有很高的灵敏度,并且在己二烯雌酚质量浓度为500~5000 ng/mL的范围内具有良好的线性关系,检测限可达到0.2 ng/mL。该传感器具有良好的稳定性和重复性,对猪肉实际样品进行了己二烯雌酚的含量分析,回收率在83.8%~97.7%之间,结果令人满意。An uhrasensitive electrochemical immunosensor modified with graphene and dienestrol （DE） was developed for the detection of dienestrol through indirect competition with K3Fe （CN） 6 acting as the redox probes. The results revealed that under optimized conditions a calibration for DE was obtained with a linear range of 500 -5 000 ng/mL and the detection limit was up to O. 2 ng/mL, showing that the proposed electrochemical immunosensor had excellent sensitivity and wide detection range. The immunosensor was examined in real samples for the analysis of DE. A good recovery in the range of 83.8% -97.7% was obtained in pork samples.

关 键 词：石墨烯 电化学免疫传感器 间接竞争 己二烯雌酚 猪肉 含量测定 

分 类 号：R155.5[医药卫生—营养与食品卫生学] TP212.3[医药卫生—公共卫生与预防医学]