不同培养基配方对北虫草中蛋白质和氨基酸含量的影响  

Effect of Different Culture Medium Formula on the Content of Protein and Amino Acid in Cordyceps militaris

作　　者：刘芳[1,2] 李帅[1] 高剑[1] 杨传伟[1] 于爽[1] 

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011 [2]牡丹江林业科学研究所,黑龙江牡丹江157009

出　　处：《黑龙江农业科学》2015年第12期15-18,共4页Heilongjiang Agricultural Sciences

基　　金：黑龙江省教育厅面上资助项目(12541838)

摘　　要：为了提高北虫草的营养价值,通过不同培养基配方培养北虫草,用凯氏定氮法和氨基酸自动分析仪测定各配方中蛋白质和氨基酸含量,分析不同培养基配方对北虫草中蛋白质和氨基酸含量的影响。结果表明:配方C(大米40g,1号营养液56mL)中蛋白质平均含量最高,为20.430g·(100g)-1。配方A(玉米40g,1号营养液56 mL)中必需氨基酸含量、非必需氨基酸含量、总氨基酸含量均最高,分别为3.882,12.512,16.394g·(100g)-1。Cordyceps militaris was cultivated by different culture medium formulation in order to improve its nutritional value. Analyzing the content of protein and amino acid in Cordyceps militaris which determined by kjeldahl method and amino acid auto analyzer. The results suggest that the average content of protein in the formul.ation C（40 g of rice,56 mL of nutrient solution 1） was the highest in the six formulations,and the average content was 20. 430 g per 100 g. The highest content of essential amino acids,non-essential amino acid and total amino acids is formulation A（40 g of corn,56 mL of nutrient solution 1）,to 3. 882 g per 100 g,12. 512 g per 100 g and 16. 394 g per 100 g respectively.

关 键 词：北虫草 培养基配方 蛋白质 氨基酸 

分 类 号：S567.35[农业科学—中草药栽培]