桃拟茎点霉对孕酮的生物转化研究  

Biotransformation of progesterone by Phomopsis amygdali

作　　者：杜刚[1] 王忠诚[1] 杨红梅[2] 张广求[1] 杨海英[2] 

机构地区：[1]云南民族大学民族药资源化学国家民族事务委员会教育部重点实验室,云南昆明650500 [2]云南民族大学化学与环境学院,云南昆明650500

出　　处：《中国酿造》2015年第12期93-96,共4页China Brewing

基　　金：云南省教育厅科学研究基金重点项目(2013Z038;2015Z111);国家自然科学基金资助项目(21462051)

摘　　要：研究了一株桃拟茎点霉(Phomopsis amygdale)对孕酮的生物转化,并对该转化菌株的形态学和分子生物学进行鉴定,转化产物经分离纯化后,运用核磁共振(NMR)、质谱(MS)、红外光谱(IR)分析进行结构鉴定。该菌株转化孕酮得到2个转化产物,转化产物分别为15β-羟基孕酮和6β,15β-二羟基孕酮。结果表明,桃拟茎点霉具有转化甾体的能力,对孕酮进行生物转化的主要产物是15β-羟基甾体衍生物。Progesterone was transformed by Phomopsis amygdale, whichwere identified with morphology and molecular biology. The transformed products were separated and purified, and their structures were identified by nuclear magnetic resonance, mass spectrum and infrared spectroscopy analysis. Progesterone was transformed by the strain into 15β-hydroxy progesterone and 6β-,15β-dihydroxy progesterone. The results showed that P. amygdale had the ability of steroid transformation, and the main biotransformation products from progesterone were 15β-hydroxy steroidal derivatives.

关 键 词：生物转化 桃拟茎点霉 孕酮 鉴定 

分 类 号：Q815[生物学—生物工程]