鲫鱼卵唾液酸糖肽对成骨细胞MC3T3-E1增殖、分化、矿化及OPG/RANKL基因表达的影响  

Effects of Sialyglycopeptides from the Eggs of Carassius auratus on the Proliferation,Differentiation, Mineralization, OPG/RANKL Gene Experssion of MC3T3-E1 Cells

作　　者：贺敏[1] 周晓春[1] 陈鹏[1] 张昕[1] 王玉明[1] 薛长湖[1] 王静凤[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国食品学报》2015年第11期16-22,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(No.31371876)

摘　　要：目的:研究鲫鱼卵唾液酸糖肽(Ca-SGPP)对成骨细胞MC3T3-E1骨形成活性及OPG/RANKL基因表达水平的影响。方法:在MC3T3-E1细胞中加入不同浓度的Ca-SGPP,采用MTT法、试剂盒和茜素红染色法检测Ca-SGPP对MC3T3-E1细胞增殖、分化和矿化的影响;利用逆转录聚合酶链式反应(RT-PCR)技术检测MC3T3-E1细胞骨形态发生蛋白-2(BMP-2)、骨保护素(OPG)及核因子κB受体活化因子配体(RANKL)基因m RNA表达水平。结果:Ca-SGPP可显著促进MC3T3-E1细胞增殖及ALP、OCN和COL I的分泌,提高MC3T3-E1细胞矿化能力,上调其BMP-2表达水平及OPG/RANKL的比值,且分子质量大的糖肽效果更佳。结论 :CaSGPP具有促进MC3T3-E1细胞增殖、分化和矿化的能力,其机制可能与OPG/RANKL比值上调有关。Objective： To investigate the effects of sialyglycopeptides from the eggs of Carassius auratus（Ca-SGPP）on the proliferation, diferentiation, mineralization and the gene expression of OPG/RANKL in MC3T3-E1 cells. Method：MTT assay, alkaline phosphatase（ALP） activity assay and calcium deposition were determined, the secretion of collagen I（COL I） and osteocalein（OCN） were assayed by enzyme-linked immunosorbent assay（ELISA）, the m RNA expression levels of bone morphogenetic protein 2（BMP-2）, osteoprotegerin（OPG） and receptor activator for nuclear factor-κB ligand（RANKL） were also measured by reverse transcription polymerase chain reaction（RT-PCR）. Results： The Ca-SGPP could effectively promote the proliferation（P〈0.01）, increase ALP,OCN and COL I content, enhance the mineralization ability, improve the expression levels of BMP-2 and OPG/RANKL ratio of MC3T3-E1 cells. Furthermore, the effect of Ca-SGPP of more than 10 ku was much better than that of less than 10 ku. Conclusion： The Ca-SGPP can remarkably promote the osteogenic activity on MC3T3-E1 cells, and its mechanism is probably related to the rise of OPG/RANKL rato.

关 键 词：唾液酸糖肽 MC3T3-E1 骨形成 OPG/RANKL 鲫鱼 

分 类 号：R580[医药卫生—内分泌]