美丽短芽胞杆菌Brevibacillus formosus FJAT-10011抑菌效应及其产蛋白酶条件优化  

Antibacterial Efficacy and Optimized Protease-producing Capability of Brevibacillus formosus FJAT-10011

作　　者：李安琪[1,2] 蓝江林[2] 刘波[2] 黄素芳[2] 王小英[1,2] 

机构地区：[1]福州大学生物科学与工程学院,福建福州350108 [2]福建省农业科学院农业生物资源研究所,福建福州350003

出　　处：《福建农业学报》2015年第10期958-964,共7页Fujian Journal of Agricultural Sciences

基　　金：国家科技支撑计划项目(2012BAD14B15);福建省科技重大专项(2012NZ0002);福建省科技计划项目--省属公益类科研院所基本科研专项(201303094;2014R1018-2)

摘　　要：对筛选到产蛋白酶且抑菌的美丽短芽胞杆菌Brevibacillus formosus FJAT-10011通过单因素和正交试验对该菌株发酵条件进行优化。优化培养基组成:可溶性淀粉1.25%、蛋白胨1.25%、牛肉浸粉0.3%、NaCl0.5%、硫酸镁0.05%、磷酸氢二钾0.05%。优化发酵条件为:发酵初始pH 5.5、发酵温度25℃、发酵培养基装液量30mL/250mL、种子液接种量1%、发酵时间24h。在优化条件下,上清液酶活力达到8.41U·mL-1,是初始培养条件的141倍。抑菌直径为15.71mm,比未优化的提高35.31%。Antibacterial efficacy andprotease-producing capability of Brevibacillus formosus FJAT-10011 were studied.Screening and optimizing the culture conditions were conducted with a single-factor test and an orthogonal experiment.The optimal medium was determined to contain 1.25% starch,1.25% peptone,0.3% beef extract powder,0.5% NaCl,0.05% MgSO4,and 0.05% K2 HPO4.The optimized fermentation process included the following conditions：an initial pH of 5.5,a temperature at 25℃,the filling of the liquid medium at 30 mL/250 mL,a bacterial inoculum rate at 1%,and a fermentation duration of 24 h.Under these conditions,the protease activity of the supernatant of the liquid medium reached 8.41U·mL-1,which was 141 times greater than that in the beginning of the fermentation.The diameter of the antibacterial zone shown on a petri dish test was 15.71 mm,a 35.31%increase as compared to the non-optimized control.

关 键 词：蛋白酶 抑菌 发酵优化 单因素 正交试验 

分 类 号：Q939.9[生物学—微生物学]