盐酸催化水解槐角异黄酮的研究  

Hydrolysis of Fructus Sophorae Isoflavones by Hydrochloric Acid Catalysis

作　　者：张晓松[1] 张博雅[1] 金花[1] 张永忠[1] 

机构地区：[1]东北农业大学应用化学系,哈尔滨150030

出　　处：《天然产物研究与开发》2015年第12期2095-2100,共6页Natural Product Research and Development

摘　　要：利用盐酸甲醇溶液对槐角总异黄酮进行水解制备染料木黄酮。采用高效液相色谱法检测槐角异黄酮水解率。探讨水解时间、盐酸浓度和水解温度对异黄酮水解率的影响,并通过响应面法确定最佳水解条件。实验结果表明最佳水解条件为:水解时间3.8 h、盐酸浓度2.59 mol/L、水解温度78.5℃。在此工艺下,槐角异黄酮水解率达93.38%。Fructus Sophorae isoflavones were hydrolyzed to genistein in hydrochloric acid-methanol solution. The hydrolysis rate of Fructus Sophorae isoflavones was detected by high performance liquid chromatography. The effects of hydrolysis time,hydrolysis temperature and hydrochloric acid concentration on hydrolysis rate of Fructus Sophorae isoflavones were investigated. The optimal hydrolysis conditions were determined as follows：hydrolysis time 3.8 h, hydrochloric acid concentration 2.59 mol/L, hydrolysis temperature 78.5 ℃. Under these conditions, the hydrolysis rate of total isoflavones reached 93.38%.

关 键 词：槐角异黄酮 酸水解 高效液相色谱 

分 类 号：Q946.8[生物学—植物学]