mTOR及PTEN、PHLPP、VEGF、p53蛋白在结肠癌中的表达及意义  

Expressions and significance of mTOR,PTEN,PHLPP,VEGF and p53 proteins in colon cancer

作　　者：张利[1] 吴慧丽[1] 李琨琨[1] 肖兴国[1] 张洋[1] 

机构地区：[1]郑州大学附属郑州中心医院消化一科,河南郑州450007

出　　处：《中国临床研究》2015年第12期1567-1569,共3页Chinese Journal of Clinical Research

基　　金：河南省科技攻关计划项目(132102310156);郑州市科研项目(121PPTGG504-7)

摘　　要：目的检测结肠癌组织中m TOR、PTEN、PHLPP及VEGF和p53蛋白表达,探讨结肠癌发生、发展可能的分子机制及临床意义。方法选择2011年3月至2012年10月65例结肠癌癌组织标本及25例腹部外伤者的正常结肠组织标本,分别采用免疫组化法及Western-blot方法检测m TOR及PTEN、PHLPP、VEGF、p53蛋白的阳性表达率及相对表达量,并对检测结果进行比较。结果 m TOR、PTEN、PHLPP、VEGF、p53蛋白在正常结肠组织中的阳性率分别为12.0%、96.0%、92.0%、12.0%、8.0%,在结肠癌组织中综合阳性率为78.5%、23.1%、20.0%、92.3%、67.7%。结肠癌组m TOR、VEGF、p53阳性表达率高于正常对照组,PTEN、PHLPP低于正常对照组(P均<0.01)。Western-blot检测结果显示,TNM分期越晚,m TOR蛋白及VEGF表达水平越高(P<0.05,P<0.01),PTEN、PHLPP蛋白表达水平越低(P<0.05,P<0.01),而p53蛋白表达水平在肿瘤TNM不同分期无明显变化。结论 m TOR通路蛋白及抑癌基因PTEN、PHLPP、p53可能参与结肠癌发生、浸润、转移的信号传导。提示m TOR通路蛋白抑制剂可用于结肠癌的治疗。Objective To study the expressions of mammalian target of rapamycin（ m TOR）,phosphatase and tensin homolog deleted on chromosome ten（ PTEN）,pleckstin homology domain leucine-rich repeat protein phosphatase（ PHLPP）,vascular endothelial growth factor（ VEGF） and p53 proteins in colon cancer tissues and discuss the clinical significance and potential molecular mechanism of occurrence and development of colon cancer. Methods The colon cancer tissue samples of 65 colon cancer patients from March 2011 to October 2012 and normal colon tissues samples of 25 patients with abdominal trauma were collected. The positive expression rates and relative expression quantities of m TOR,PTEN,PHLPP,VEGF,p53 proteins in tissues were detected by immunohistochemical method and Western-blot method,respectively,and the results were compared between two groups. Results The positive expression rates of m TOR,PTEN,PHLPP,VEGF and p53 proteins in normal colon tissues were 12. 0%,96. 0%,92. 0%,12. 0%,8. 0%,respectively,and were 78. 5%,23. 1%,20. 0%,92. 3% and 67. 7%,respectively in colon carcinoma tissues. The expressions of m TOR,VEGF and p53 proteins in colon cancer tissue significantly higher than those in normal colon tissues（ all P 0. 01）,while the expressions of PTEN and PHLPP in colon cancer tissues were significantly lower than those of normal colon tissue（ all P 0. 01）. Western-blot test showed that the later TNM stage,the higher expressions of m TOR and VEGF proteins（ P 0. 05 or P 0. 01） and the lower expressions of PTEN and PHLPP in colon cancer tissues（ P 0. 05 or P 0. 01）,while there was no obvious changes of p53 protein expression in different TNM stages. Conclusions The m TOR signaling pathway protein and PTEN,PHLPP and p53 tumor suppressor genes might participate in the signal transduction of occurrence,invasion,metastasis of colon cancer. It is prompted that the inhibitors of m TOR pathway proteins can be used for the therapy of colon cancer.

关 键 词：MTOR信号通路 结肠癌 血管内皮生长因子 PTEN基因 PHLPP基因 P53蛋白 抑癌基因 

分 类 号：R735.3[医药卫生—肿瘤]