拉布拉多犬髋关节发育不良相关基因的SNP位点的检测  

Detection for SNPs of Canine Hip Dysplasia(CHD) Related Genes in Labrador Retrievers

作　　者：赵明媛[1] 韩芳[1] 李雅婵[1] 俞剑熊 张雅丽[1] 周子娟[1] 王福金[1] 王爱国[1] 王靖宇[1] 

机构地区：[1]大连医科大学实验动物中心,大连116044

出　　处：《实验动物科学》2015年第4期21-25,共5页Laboratory Animal Science

基　　金：国家自然科学基金(No.31272392)

摘　　要：目的检测拉布拉多犬基因组DNA中与髋关节发育不良密切相关的5个基因中的6个SNP位点,以期为拉布拉多犬的选育提供有效的遗传学诊断方法。方法经影像学诊断,筛选出典型的6只髋关节发育不良和11只正常拉布拉多犬作为实验对象,采取静脉血,提取血细胞的基因组DNA样本,采用PCR/测序方法对17个样本的5个基因中的6个SNP位点:LAMA2(CFA1:70997779),LRR1(CFA8:29247021),SCR(CFA24:28944481),COL6A3(CFA25:51031100,51040259)和FN1(CFA37:25095511)进行变异型检测,并统计分析SNP位点的变异型与髋关节发育不良犬关联度。结果 LAMA2(CFA1:70997779),LRR1(CFA8:29247021),SCR(CFA24:28944481),COL6A3(CFA25:51031100)和FN1(CFA37:25095511)SNP位点的变异型与拉布拉多犬的髋关节发育不良没有显著相关性(P>0.05)。COL6A3(CFA25:51040259)SNP位点的A/A型与拉布拉多犬的髋关节发育不良存在极显著相关性(P=0.007)。结论 COL6A3(CFA25:51040259)SNP位点的A/A型可能成为拉布拉多犬髋关节发育不良的实用性遗传学诊断指标,但尚需大样本确认。Objective To provide an efficient genetic diagnostics method for Labrador Retrievers,6 SNPs of 5canine hip dysplasia（ CHD） related genes in genomic DNA of Labrador Retrievers were detected. Method 11 normal and 6 typical CHD Labrador Retrievers were selected by imaging diagnosis. The vein blood was sampled and the genomic DNA was isolated from white blood cells. The PCR / sequencing method was used to analyze the variants of 6 SNPs in 5 canine hip dysplasia（ CHD） related genes（ LAMA2（ CFA1： 70997779）,LRR1（ CFA8：29247021）,SCR（ CFA24： 28944481）,COL6A3（ CFA25： 51031100, 51040259）, and FN1（ CFA37：25095511））. Furth more,the association between the SNP variants and CHD was analyzed by Chi-square Test.Result The variants of LAMA2（ CFA1： 70997779）,LRR1（ CFA8： 29247021）,SCR（ CFA24： 28944481）,COL6A3（ CFA25： 51031100）,and FN1（ CFA37： 25095511） SNP loci were not significantly associated to CHD（ P 0. 05）. A/A variants in COL6A3（ CFA25： 51040259） SNP locus was significantly associated to CHD（ P =0. 007）. Conclusion A / A variants in COL6A3（ CFA25： 51040259） SNP locus might be used as a practical genetic detection index for CHD diagnostics in Labrador Retrievers. However,further confirmation in large-sample is required.

关 键 词：拉布拉多犬 髋关节发育不良(CHD) 单核苷酸多态性(SNP) 遗传学诊断 

分 类 号：R394.8[医药卫生—医学遗传学]