Potential of the White-Rot Fungus Pleurotus pulmonarius F043 for Degradation and Transformation of Fluoranthene  

作　　者：Riry WIRASNITA 

机构地区：[1]Centre for Environmental Sustainability and Water Security(IPASA),Research Institute for Sustainable Environment,UniversitiTeknologi Malaysia,81310 UTM Skudai,Johor Bahru(Malaysia) [2]Department of Environmental Engineering,Faculty of Civil Engineering,Universiti Teknologi Malaysia,81310 UTM Skudai,JohorBahru(Malaysia)

出　　处：《Pedosphere》2016年第1期49-54,共6页土壤圈（英文版）

基　　金：supported by the Fundamental Research Grant Scheme(FRGS)of Ministry of Education,Malaysia(No.R.J130000.7809.4F465;the Science Fund of Ministry of Science,Technology and Innovation,Malaysia(No.R.J130000.7909.4S110)

摘　　要：Fluoranthene, a four-ring polycyclic aromatic hydrocarbon that is possible genotoxic in nature, has been used as an indicator for assessing polycyclic aromatic hydrocarbon （PAH）-containing pollutants. Microbial degradation is one of the promising methods in removing up PAH-contaminated environments. White-rot fungi have showed the ability to degrade a wide range of PAHs. This study aimed to investigate enzyme production, fungal biomass, and glucose utilization during the biodegradation process of fiuoranthene by a white-rot fungus Pleurotus pulmonarius F043 and to identify the metabolites produced in the degradation process. The extracellular ligninolytic enzyme system of the fungi, producing laccases and peroxidases, was directly linked to the biodegradation of fiuoranthene. The production of ligninolytic enzymes during fluoranthene degradation was related to an increase in the biomass of Pleurotus pulmonarius F043. Fluoranthene removal decreased with an increase in fluoranthene concentrations. The highest biomass production of Pleurotus pulmonarius F043 （〉 4 400 mg L-1） was found in the 10 mg L-1 fluoranthene culture after 30 d of incubation. Two fluoranthene metabolites, naphthalene-l,8-dicarboxylic acid and phthalic acid, were found in the process of fluoranthene degradation. Laccase was revealed as the major enzyme that played an important role in degradation process. Suitable conditions must be found to promote a successful fungal biotransformation augmentation in liquid culture.Fluoranthene, a four-ring polycyclic aromatic hydrocarbon that is possible genotoxic in nature, has been used as an indicator for assessing polycyclic aromatic hydrocarbon(PAH)-containing pollutants. Microbial degradation is one of the promising methods in removing up PAH-contaminated environments. White-rot fungi have showed the ability to degrade a wide range of PAHs. This study aimed to investigate enzyme production, fungal biomass, and glucose utilization during the biodegradation process of fluoranthene by a white-rot fungus Pleurotus pulmonarius F043 and to identify the metabolites produced in the degradation process. The extracellular ligninolytic enzyme system of the fungi, producing laccases and peroxidases, was directly linked to the biodegradation of fluoranthene.The production of ligninolytic enzymes during fluoranthene degradation was related to an increase in the biomass of Pleurotus pulmonarius F043. Fluoranthene removal decreased with an increase in fluoranthene concentrations. The highest biomass production of Pleurotus pulmonarius F043(≥ 4 400 mg L^(-1)) was found in the 10 mg L^(-1) fluoranthene culture after 30 d of incubation. Two fluoranthene metabolites, naphthalene-1,8-dicarboxylic acid and phthalic acid, were found in the process of fluoranthene degradation.Laccase was revealed as the major enzyme that played an important role in degradation process. Suitable conditions must be found to promote a successful fungal biotransformation augmentation in liquid culture.

关 键 词：gas chromatography-mass spectrometer ligninolytic enzymes METABOLITES naphthalene-l 8-dicarboxylic acid phthalicacid 

分 类 号：X172[环境科学与工程—环境科学] X788