应用T-RFLP技术分析鲈鱼肠道菌群  

Analysis of bacterial community in the intestinal of Lateolabrax japonicus using T-RFLP technique

作　　者：潘艳艳[1] 钱云霞[1] 张德民[1] 

机构地区：[1]宁波大学海洋学院,宁波315211

出　　处：《生物学杂志》2015年第6期100-104,共5页Journal of Biology

基　　金：国家高技术研究发展计划(863计划):海洋生态环境高通量生物检测技术开发(2012AA092001)

摘　　要：鱼类肠道菌群在鱼类的消化、吸收、免疫反应以及器官的发育等方面起着重要作用。为确定鲈鱼肠道不同部位和粪便的主要细菌类型与群落结构,运用末端限制性片段长度多态性(T-RFLP)技术分析了鲈鱼肠道壁的前肠壁、中肠壁、后肠壁及粪便中的细菌群落特征及多样性。结果表明:粪便的菌落结构区别于肠道壁,其多样性最高;变形菌是鱼类最重要的肠道菌,尤其是γ-变形菌纲普遍存在于鲈鱼肠道壁的不同部位与粪便中;后肠壁组以T-RF493 bp所代表的不动杆菌属(Acinetobacter)为最优势菌,T-RF 488 bp所代表的丛毛单胞菌属(Comamonas)则同时为中肠壁组和前肠壁组的最优势菌,而T-RF 434 bp所代表菌为粪便最优势菌。Microflora in fish digestive tract play important roles in digestion, absorption, immunity and organ development. In order to assess the dominant bacterial composition along the intestinal tract of Lateolabrax japonicus, terminal restriction fragment length poly-morphism （ T-RFLP） technique was used to analyze the characteristics and diversity of bacterial communities in three parts of gut （ foregut, midgut and hindgut） and feces. Clustering analysis based on the T-RFLP profiles showed that there were some compositional differences in bacterial communities. Further analysis of T-RFLP profiles with clone library technique showed that Proteobacteria, espe-cially γ-Proteobacteria, was the most prominent phylum in different parts of gut and feces. Two dominant genus of γ-Proteobacteria, Acinetobacter （ T-RF 493 bp） and Comamonas （ T-RF 488 bp） , displayed different abundance in different parts of gut and feces;Acin-etobacter was the most dominant bacteria in hindgut;Comamonas was the most dominant bacteria in both foregut and midgut, while the dominant bacteria in feces was represented by T-RF 434 bp.

关 键 词：鲈鱼 细菌群落 肠道 T-RFLP 

分 类 号：S917.1[农业科学—水产科学]