番茄SlMYB12 RNAi表达载体构建及功能的初步研究  

Construction of SlMYB12 RNAi expression vector and functional analysis in tomato

作　　者：刘琳琳[1] 田荣荣[2] 郭萧[1] 廖雄[1] 王爽[1] 姚丽萍[1] 赵迪[1] 王彦涛[1] 李天红[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]北京城市学院生物医药学部,北京100194

出　　处：《中国农业大学学报》2015年第6期117-123,共7页Journal of China Agricultural University

基　　金：国家自然科学基金(31171938);公益性行业(林业)科研专项经费(201404720)

摘　　要：以Micro-Tom番茄(Solanum lycopersicum L)为材料,构建SlMYB12 RNAi载体,利用农杆菌介导法导入番茄;对转基因植株评价发现,SlMYB12基因被干扰后植株出现了植株矮化、果实变小、种子数减少和转色期提前等变化;实时荧光定量PCR检测表明干扰植株果实中类黄酮路径相关基因SlCHS、SlF3H、SlF3’H表达量下降,SlCHI基因表达量上升。Micro-Tom tomato（Solanum lycopersicum L.）was used as material in this study.The SlMYB12 RNAi expression vector was constructed,and introduced into tomato via Agrobacterium tumefaciens mediated method.PCR results showed that the gene was integrated in tomato.The transgenic lines exhibited dwarf phenotype,smaller fruits and advanced mature period.The results of qRT-PCR showed that the expression of flavonoid biosynthesis-related genes SlCHS、SlF3H、SlF3＇H were decreased in transgenic lines,while the expression of SlCHI gene was increased.These results provided an important basis for further study of SlMYB family genes function.

关 键 词：番茄 类黄酮 SlMYB12 RNAI 实时荧光定量PCR 

分 类 号：S641.2[农业科学—蔬菜学]