DNA条形码序列对药食两用品花椒的品种鉴定  

Authentication of homology of medicine and food Huajiao by DNA barcoding sequences

作　　者：王福[1] 闫珂巍 梅国荣[1] 卢俊宇[1] 潘欢欢[1] 陈鸿平[1] 陈林[1] 何玉琼[1] 刘友平[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137

出　　处：《食品工业科技》2016年第1期138-141,共4页Science and Technology of Food Industry

基　　金：2015版<中国药典>增修订项目

摘　　要：目的：利用DNA条形码鉴定技术,快速、准确的鉴别中药花椒,区别中药花椒及其市售香料花椒的品种来源差异。方法：采用植物DNA试剂盒提取花椒及其混伪品基因组DNA,对全部收集样品的ITS2序列进行扩增和测序,计算物种种间种内Kimura2-parameter（K2P）遗传距离。采用Blast、最近距离法及基于ITS2序列构建Neighbor-joining（NJ）系统聚类树进行鉴别分析。结果：中药花椒及香料花椒的序列长度为224~227 bp,中药花椒种间平均K2P遗传距离明显大于香料花椒种内平均K2P遗传距离;ITS2分子系统树显示,中药花椒及香料花椒均聚为一单系分枝。结论：ITS2序列作为DNA条形码能准确鉴别中药花椒及其香料花椒,为中药花椒及其香料花椒的鉴别提供了依据。Objective： To authenticate Pericarpium Zanthoxyli and investigate the origin of Pericarpium Zanthoxyli and commercial spice peppers accurately,DNA barcoding technique was used. Method： Total genomic DNA was isolated from Pericarpium Zanthoxyli and its adulterants using the plant DNA kit.The ITS2 sequences of all samples were amplified and sequenced. The Kimura 2- parameter（ K2P） distances were calculated. Identification analyses were performed using Blast,nearest distance and Neighbor- joining（ NJ） methods. Result： The lengths of Pericarpium Zanthoxyli and commercial spice peppers were between 224 ~ 227 bp. Interspecific distances of Pericarpium Zanthoxyli was bigger than the intraspecific distances of commercial spice peppers. The NJ tree showed that every breeds had a single branches.Conclusion： The sequence of ITS2 was efficient barcode for the authentication of Pericarpium Zanthoxyli and other spices,and provided according for the identification of Pericarpium Zanthoxyli and commercial spice peppers.

关 键 词：中药花椒 ITS2 PSBA-TRNH 分子鉴定 DNA条形码 

分 类 号：TS201.3[轻工技术与工程—食品科学]