茶提取物和纳米表没食子儿茶素没食子酸酯对6-羟基多巴胺诱导的SH-SY5Y细胞保护作用  

Protective Effects of Tea Extracts and Nano-EGCG against 6-OHDA-Induced Cell Injury in SH-SY5Y Cells

作　　者：闫敬娜 罗理勇[1,2] 胡雅琼[1,3] 曾亮[1,2] 

机构地区：[1]西南大学食品科学学院,重庆400715 [2]西南大学茶叶研究所,重庆400715 [3]中国民生银行黄岩支行,浙江台州318020

出　　处：《食品科学》2016年第1期163-170,共8页Food Science

基　　金：国家自然科学基金青年科学基金项目(31100500);重庆市科委自然科学基金计划项目(CSTC.2013jcyjA80021);中央高校基本科研业务费专项资金项目(XDJK2013B036)

摘　　要：以6-羟基多巴胺(6-hydroxydopamine,6-OHDA)诱导的SH-SY5Y细胞系作为帕金森病(Parkinson’s disease,PD)体外细胞模型,探究茶提取物(L-茶氨酸、咖啡碱、茶多酚、表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate,EGCG)、茶黄素、茶色素)和两种纳米EGCG对SH-SY5Y细胞的毒性和对6-OHDA诱导的SH-SY5Y细胞保护作用的差异,综合评价茶提取物治疗PD的潜力。结果表明:茶提取物毒性作用由弱到强为茶多酚<L-茶氨酸<茶色素<咖啡碱<茶黄素<EGCG;保护作用由强到弱为茶黄素>EGCG>茶多酚>茶色素>咖啡碱>L-茶氨酸;茶黄素(0.2μg/m L)和EGCG(0.9μg/m L)预处理6-OHDA诱导的SH-SY5Y细胞,细胞存活率分别为(99.59±2.24)%和(95.84±2.50)%,保护作用明显强于其他茶提取物;相较于EGCG,纳米EGCG具有毒性作用更弱和保护作用更强的特点。由此可知,茶作为一种日常饮品,具有较好地治疗PD的潜力。Human neuroblastoma cell line SH-SY5 Y challenged with 6-hydroxydopamine（6-OHDA） was used as an in vitro model of Parkinson＇s disease（PD）, and the cytotoxicity of tea extracts including L-theanine, caffeine, tea polyphenol, epigallocatechin-3-gallate（EGCG）, theaflavins, tea pigment, and two kinds of nano-EGCG on SH-SY5 Y cells were investigated, and their protective effects on 6-OHDA-induced SH-SY5 Y cells were compared to evaluate their potentials for the treatment of PD. The results showed that the cytotoxicity was in the following order： tea polyphenol L-theanine tea pigment caffeine theaflavins EGCG; the protective effects were in the following order： theaflavins EGCG tea polyphenol tea pigment caffeine L-theanine. Theaflavins（0.2 μg/m L） and EGCG（0.9 μg/m L） were more effective than other tea extracts in preventing SH-SY5 Y cells from 6-OHDA-induced damage, restoring cell viability to（99.59 ± 2.24）%and（95.84 ± 2.50）%, respectively. Compared to EGCG, nano-EGCG had weaker cytotoxicity and stronger protective effect. Based on our results, tea, as a natural daily drink, has better potential for the treatment of PD.

关 键 词：帕金森病 茶提取物 6-羟基多巴胺 SH-SY5Y细胞 纳米表没食子儿茶素没食子酸酯 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]