生态修复工程对城市内河水体细菌多样性的影响  

Impacts of ecological restoration treatments on diversity of prokaryotic bacteria in urban river

作　　者：唐方园[1] 张秋芳[1] 周阳靖[1] 陈力行 徐继荣[1] 程军蕊[1] 

机构地区：[1]宁波大学建筑工程与环境学院,宁波315211 [2]宁波市城管局内河处,宁波315211

出　　处：《环境工程学报》2016年第1期445-454,共10页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(41571252);浙江省公益性技术应用研究计划项目(2012C23065);浙江省自然科学基金项目(Y5110331);宁波市自然科学基金项目(2011A610085);宁波市社会发展项目(2011C50050)资助

摘　　要：微生物是城市内河生态系统的重要组成部分,其多样性与水环境密切相关。以16S rRNA基因作为原核细菌分子标记,应用PCR-DGGE、定量PCR、克隆和测序方法,分析经生态修复工程处理后不同污染程度的城市内河水体细菌多样性特征。结果表明:生态修复工程明显提高了污染程度较低水体总氮(TN)、总磷(TP)和化学需氧量(CODMn)的消减能力,影响着城市内河水体中细菌群落的组成和含量,且随时间和空间不同而不同;生态修复工程显著地提高了重度污染水体细菌的含量;冗余分析(RDA)表明,水体中细菌群落组成与CODMn、TP和溶解氧(DO)等指标密切相关。Microorganism is of significance in the ecosystem of urban river and its diversity is closely related to the water environment. In order to explore the impacts of ecological restoration treatments on the prokaryotic bacteria in urban rivers,the methods of PCR-denaturing gradient gel electrophoresis（ DGGE）,real-time quantitative PCR（ q PCR）,cloning and sequencing were used to detect the composition and quantity of 16 S rRNA gene. The results showed that the higher ability of reducing total nitrogen（ TN）,total phosphorus（ TP） and chemical oxygen demand（ CODMn） was promoted by the ecological restoration treatments in the cleaner surface waters than those of others. The composition and abundance of bacterial 16 S rRNA gene were spatio-temporally different after the restoration treatment. Especially,the quantity of prokaryotic bacteria was improved dramatically in the waters of seriously polluted zones than those of others. The redundancy analysis（ RDA） revealed that the bacterial composition was significantly related to CODMn,TP and dissolved oxygen（ DO） in the urban river.

关 键 词：16S RRNA 基因 生态修复 城市内河 水质 

分 类 号：X172[环境科学与工程—环境科学]