用壳聚糖修饰的大孔SiO_2材料固定化脂肪酶拆分萘普生甲酯  

Chiral Resolution of Racemic Naproxen Methyl Esters Catalyzed by Lipase Immobilized on Macroporous SiO_2 Coated Chitosan

作　　者：龙能兵[1] 孙怀艳[1] 李伟逊[1] 

机构地区：[1]宁波大学材料科学与化学工程学院,浙江宁波315211

出　　处：《广东化工》2016年第1期8-9,5,共3页Guangdong Chemical Industry

基　　金：宁波市自然科学基金项目(2013A610086);宁波大学科研基金项目(XYL13005)

摘　　要：以壳聚糖(Chitosan,CS)修饰的大孔SiO_2材料为载体,通过吸附法固定柱状假丝酵母菌脂肪酶(Candida rugosa lipase,CRL)获得了CRL/CS-SiO_2固定化脂肪酶。并以催化拆分萘普生甲酯水解反应为对象,研究大孔SiO_2载体性能以及CRL固定量对反应的影响。结果表明,该CRL/CS-SiO_2固定化酶在结构上仍然保持载体Si O_2材料的微米级孔道,且CRL以纳米薄层的形式较为均匀地涂覆在孔道表面上。该固定化酶催化生成(S)-萘普生的转化率和选择性都远高于游离酶,其产物转化率随着CRL酶固定量的增加而增大,当CRL负载量为53.2 mg/g,反应120 h时,(S)-萘普生的转化率可达到44.6%,对映体过剩值eep达到100%,对映体选择性远大于200。The catalytic performance of Candida rugosa lipase（CRL） immobilized on the macroporous SiO_2 coated chitosan（CS） in the transesterification resolution of racemic naproxen methyl esters was studied. The effect of SiO_2 characteristics and immobilized amount of CRL on the kinetic resolution were investigated. The results show that the CRL/CS-SiO_2 remain keep the pore sizes in the range of 1~2 μm, and CRL coated on the 3D silica layer as thin films. The conversion and enantioselectivity of（S）-naproxen of CRL/CS-Si O_2 higher than that of free CRL,（S）-naproxen production and conversion increased linearly with the amount of immobilized CRL. The conversion reached to 44.6 % after 120 hours of reaction and 100 % of enantiometric excess（eep） and 200 of enantioselectivity were obtained.

关 键 词：(S)-萘普生 CRL脂肪酶 大孔SiO2材料 手性拆分 壳聚糖 固定化脂肪酶 

分 类 号：TQ814.3[化学工程]