亲水色谱柱对氨基酸及其美拉德反应初始中间体的分离  

Separation of Amino Acids and Their Initial Maillard Reaction Intermediates by Hydrophilic Interaction Liquid Chromatography

作　　者：曹长春[1] 梁晶晶[1] 王蒙[1] 侯莉[1] 谢建春[1] 孙宝国[1] 

机构地区：[1]北京工商大学食品质量与安全北京实验室,北京市食品风味化学重点实验室,北京市食品添加剂工程技术研究中心,北京100048

出　　处：《食品科学》2016年第2期63-71,共9页Food Science

基　　金：国家自然科学基金面上项目(31371838;31171755);北京市教委科技发展计划重点项目(KZ20101001011);“十二五”国家科技支撑计划项目(2011BAD23B01)

摘　　要：采用Xbridge BEH Amide和Atlantis HILIC Silica两亲水色谱柱分离半胱氨酸、甘氨酸及其与木糖形成的美拉德反应初始中间体2-木糖基噻唑烷-4-羧酸、甘氨酸-Amadori和半胱氨酸-Amadori 5个化合物。通过研究流动相中有机相(乙腈)的比例、缓冲盐浓度、pH值、柱温、流速对容量因子和分离度的影响,分别找到较佳分离条件。在BEH Amide色谱柱上5个化合物之间的分离度均达到2.28以上,对比可知,采用BEH Amide色谱柱更好。An Xbridge BEH Amide column and an Atlantis HILIC Silica column, which work on hydrophilic interaction liquid chromatography（HILIC）, were used to separate cysteine, glycine, and their initial Maillard reaction intermediates with xylose including 2-xylulosylthiazolidine-4-carboxylic acid, glycine-Amadori, and cysteine-Amadori. The effects of the ratio of the organic phase（CH_3CN）, the concentration of ammonium formate（the buffer salt）, the pH value and flow rate of the mobile phase, and column temperature on capacity factor and resolution factor were investigated. Then optimum separation conditions for the Xbridge BEH Amide column and the Atlantis HILIC Silica column were established, respectively. Using the BEH Amide column, the resolution factors achieved for the five compounds all exceeded 2.28. The results showed that the BEH Amide column was more suitable for the chromatographic separation.

关 键 词：亲水色谱 半胱氨酸 2-木糖基噻唑烷-4-羧酸 甘氨酸 甘氨酸-Amadori 半胱氨酸-Amadori 分离 

分 类 号：TQ656.1[化学工程—精细化工]