桔梗糖蛋白的组成分析和对·OH的清除作用的研究  

Study on the composition analysis and inhibition of·OH of glycoprotein from Platycodon grandiflorum

作　　者：廉亚楠 果婷婷 崔琳[1] 赵胜男[1] 林雪燕[1] 郑晓凤[1] 高金波[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《食品工业科技》2016年第3期83-87,共5页Science and Technology of Food Industry

基　　金：佳木斯大学大学生"校长创新创业基金项目"研发项目课题(xzyf2013-07);佳木斯大学大学生科技创新项目(2014-013);佳木斯大学大学研究生科技创新项目(LM2015-095)

摘　　要：分离纯化经超临界CO_2辅助提取的桔梗糖蛋白,确定其单糖及氨基酸组成,考察其对·OH自由基的清除作用。以DEAE-52纤维素柱及Sephadex G-75柱分离纯化桔梗糖蛋白,分别利用PMP法柱前衍生和OPA-FMOC联合衍生测定糖蛋白中单糖和氨基酸的种类与组成,分光光度法检测桔梗糖蛋白对·OH自由基的清除作用。结果显示:桔梗糖蛋白主要由7种单糖及15种氨基酸组成,平均糖含量为61.82%,蛋白含量为33.11%,对·OH的清除能力的IC50值为0.99 mg/m L。由此表明:桔梗糖蛋白具有一定的抗氧化能力,为其用于功能食品、药物制造等方面发展提供理论依据。Separation and purification of glycoprotein which were extracted by super critical CO_2 from Platycodon grandiflorum.Then the monosaccharide and amino acids composition and the inhibition of ·OH of glycoprotein were investigated. The glycoprotein were extracted from Platycodon grandiflorum and purified by DEAE- 52 cellulose column and Sephadex G-75 gel column.Monosaccharide and amino acids of glycoprotein were analyzed by pre- column derivatization with PMP and OPA- FMOC respectively. The results showed that Platycodon grandiflorum glycoprotein was composed of 7 kinds of monosaccharide（ average content of 61.82%） and 15 kinds of amino acids（ average content of 33.11%）. IC_（50） value of inhibition ·OH was 0.99 mg / m L. The study showed that·OH could be inhibited by the Platycodon grandiflorum glycoprotein effectively,which provided the theoretical basis for the further study on functional food and the drugs manufacture.

关 键 词：桔梗 糖蛋白 组成分析 

分 类 号：TS201.5[轻工技术与工程—食品科学]