姜油树脂中β-倍半水芹烯的分离纯化  

Separation of β- sesquiphellandrene from ginger oleoresin

作　　者：周长远 王艳[2] 杜爱玲[3] 

机构地区：[1]枣庄市台儿庄区林业局,山东枣庄277400 [2]山东职业学院生物工程系,山东济南250104 [3]山东大学化学与化工学院,山东济南250061

出　　处：《食品工业科技》2016年第3期225-228,共4页Science and Technology of Food Industry

基　　金：科技部农业科技成果转化项目(2008GB2C600178)

摘　　要：应用超临界二氧化碳萃取姜油树脂,选用乙醇-水作流动相,HPD-100大孔树脂作固定相分离出其中的烯类物质,并用高效液相色谱法进行跟踪检测确定含烯类的洗脱段。对比不同展开剂下烯类物质在硝酸银硅胶板上的层析效果,最终选用V_(正己烷)∶V_(乙酸乙酯)=95∶5的溶剂作流动相分离其中的β-倍半水芹烯。GC/MS检测结果表明,分别选用HPD-100大孔树脂和硝酸银硅胶作为固定相可以将姜油树脂中的β-倍半水芹烯很好的分离出来,其峰面积百分数达88.73%,收率为84.05%。通过进一步HPLC检测证明β-倍半水芹烯具有紫外吸收,其特征吸收波长为232 nm。A ethanol- water solution was used as mobile phase,and the HPD- 100 macroporous resin as the stationary phase to separate the vinyl part from ginger oleoresin extracted by supercritical carbon dioxide,and HPLC was used for detecting vinyls tracing.Different developing solvents of vinyls on argentated silica gel G precoated plate were compared,V_（hexane）∶ V_（ethylacetate）= 95 ∶ 5 was determined as the mobile phase to sepatateβ- sesquiphellandrene. The GC / MS test results indicated that HPD- 100 macroporous resin and silver nitrate modified silica gel were separately used as the stationary phase which could effectively separateβ- sesquiphellandrene from ginger oleoresin with 88.73% area percentage and 84.05% recovery. Through further HPLC analysis,β- sesquiphellandrene was found its existence of UV absorption at 232 nm.

关 键 词：硝酸银硅胶 β-倍半水芹烯 分离 

分 类 号：TS201.1[轻工技术与工程—食品科学]