蔗糖及木糖醇对血链球菌和变异链球菌相互作用的影响  

The influence of sucrose and xylitol on the interaction between Streptococcus sanguis and Streptococcus mutans

作　　者：赵光洁[1] 赵燕霞[1] 姜新亚[1] 李蓓蕾[1] 楚金普[1] 刘学军[1] 

机构地区：[1]郑州大学口腔医学院,河南郑州450052

出　　处：《牙体牙髓牙周病学杂志》2016年第1期7-11,20,共6页Chinese Journal of Conservative Dentistry

摘　　要：目的:研究不同浓度的蔗糖、木糖醇对血链球菌(S.s)产生过氧化氢(H_2O_2)能力及血链球菌与变异链球菌(S.m)相互作用的影响。方法:采用邻联茴香胺-辣根过氧化物酶法测定有氧环境中不同浓度蔗糖、木糖醇对S.s产生H_2O_2的影响,通过平板培养法观察不同浓度蔗糖、木糖醇对有氧、厌氧环境中S.s、S.m相互作用的影响。结果:有氧环境下,S.s在不同浓度的蔗糖、木糖醇培养基中培养6 h累积产生H_2O_2的量由高到低BHI+50 mmol/L蔗糖组>BHI+5 mmol/L蔗糖组>BHI组>BHI+5 mmol/L木糖醇组>BHI+50 mmol/L木糖醇组(P<0.05);6 h时,菌悬液A600值标准化之后的相对H_2O_2量为BHI组>BHI+5 mmol/L蔗糖组>BHI+50 mmol/L蔗糖组>BHI+5 mmol/L木糖醇组>BHI+50 mmol/L木糖醇组(P<0.05)。同时接种两种细菌在有氧、厌氧环境均中未观察到相互抑制的作用;先接种S.m,对S.s的抑制作用在50 mmol/L蔗糖中最明显,同种培养基中厌氧环境下的抑制作用强于有氧环境;先接种S.s,对S.m的抑制作用在50 mmol/L蔗糖中最明显,同种培养基中有氧环境下的抑制作用强于厌氧环境。结论:S.s在不同含糖培养基中产生H_2O_2的能力不同;S.s与S.m的相互作用受到接种顺序、外源性糖及环境中氧的影响。AIM： To investigate the influence of different concentrations of sucrose and xylitol on the pro- duction of hydrogen peroxide by Streptococcus sanguis （ S. s） and the interaction between S. s and Streptococcus mutans （S. m）. MTHODS ： The production of H2 O2 by S. s was determined by o - Dianisidine dihydrochloride - horseradish peroxidase with different sucrose and xylitol concentrations. The interaction between S. s and S. m was observed by plat- ing method with carbohydrates in both aerobic and anaerobic environment. RESULTS：Under aerobic environment with different carbohydrates the total production of H2O2 by S. s was as following： BHI ＋ 50 mmol/L sucrose 〉 BHI ＋ 5 mmol/L sucrose 〉 BHI 〉 BHI ＋ 5 mmol/L xylitol 〉 BHI ＋ 50 mmol/L xylitol （P 〈 0.05 ）. After H2 O2 concentration being adjusted to the absorbance of the bacteria suspession at 600 nm, the result was BHI 〉 BHI ＋ 5 mmol/L sucrose 〉 BHI ＋ 50 mmol/L sucrose 〉 BHI ＋ 5 mmol/L xylitol 〉 BHI ＋ 50 mmol/L xylitol （P 〈 0.05 ）. The inhibition was not ob- served when S. s and S. m were inoculated simultaneously. The H2 O2 production inhibition was the strongest when the bacteria were culture with 50 mmol/L sucrose and stronger in anaerobic environment when S. m was inoculated first. The inhibition was the strongest with 50 mmol/L sucrose and stronger in aerobic environment when S. s was inoculated first. CONCLUSION：The H2O2 production by S. s is influenced by sucrose and xylitol concentrations . The interac- tion of S. s on S. m is influenced by inoculation time and sequences, different kind of carbohydrates and the oxygen con- eentration.

关 键 词：血链球菌 过氧化氢 蔗糖 木糖醇 变异链球菌 

分 类 号：R780.2[医药卫生—口腔医学]