用于污染黄曲霉毒素花生分选的荧光信号研究  

Fluorescent signal characteristics for sorting of peanut contaminated by aflatoxion

作　　者：王叶群[1] 杨增玲[1] 张绍英[1] 刘婷[1] 

机构地区：[1]中国农业大学工学院,北京100083

出　　处：《农业工程学报》2016年第1期187-192,共6页Transactions of the Chinese Society of Agricultural Engineering

基　　金："十二五"国家科技支撑计划资助项目(2012BAD31B09)

摘　　要：为在加工前将黄曲霉毒素超限的带衣花生米从原料中剔除,参照已有的色选系统,提出一种依据黄曲霉毒素含量超限带衣花生米的专属荧光信号进行逐粒分选的技术构想。采用Cary Eclipse荧光分光光度计测定100粒外观具有代表性的带衣花生米表面的紫外-荧光规律,通过与免疫亲和层析净化荧光光度法（GB/T18979-2003）检测结果对比,判定了黄曲霉毒素超限带衣花生米的荧光光谱特征;通过绘制450/490、460/490荧光强度比值的箱线图,评估了表面荧光法判断黄曲霉毒素超限带衣花生米的准确率;在搭建的荧光成像系统上,对黄曲霉毒素超限带衣花生米进行了荧光成像。检测发现,在365 nm波长激发下,黄曲霉毒素超限带衣花生米在420~460 nm处有荧光峰;以450/490荧光强度比值为依据剔除超限值带衣花生米的判断准确率为81%;a.u.〉40的带衣花生米可在图像中呈现亮蓝荧光光斑。表明表面荧光信号可作为带衣花生米在线、无损、逐粒分选的专属光学信号,用于黄曲霉毒素超限带衣花生米的剔除。Peanuts are especially susceptible to contamination of aspergillusflavus and aspergillusparasiticus, which can produce a kind of highly toxic substance, aflatoxin. The study about the separation technology of aflatoxin-infected peanuts before processing can effectively isolate the source of contamination by taking precautions instead of executing treatment on the contamination, thus being of great significance on guaranteeing food safety. For the purpose of effectively eliminating the red-skin peanuts with excessive aflatoxin levels from raw materials, one technology concept of one-by-one sorting method using the exclusive fluorescent signal of the red-skin peanuts with excessive aflatoxin levels was conceived here referring to the existing color sorting system. In this study, the fluorescence spectra of 100 red-skin peanut samples with representative appearance were determined using Cary Eclipse fluorescent spectrophotometer（at an excitation wavelength of 365 nm, an emission wavelength in the range of 400 ~600 nm and discharge voltage of 400 V）. According to the fluorescence intensity distribution of samples at the wavelength of 450 nm, all these 100 red-skin peanut samples were divided into 8 groups. The aflatoxin content for each group of samples was determined using the standard method, GB/T18972-2003 Determination aflatoxin content in food-Cleanup by immunoaffinity chromatography determination by highperformance liquid chromatography and fluorimeter. By comparing the surface fluorescence spectroscopy of the 8 groups with the obtained results from the standard method, the fluorescence spectroscopy characteristics of the the red-skin peanuts with excessive aflatoxin levels were primarily determined. The accuracy rate of discriminating the aflatoxin levels of red-skin peanuts using surface fluorescence was evaluated by means of drawing the box-plots of the fluorescence intensity ratio at 450 nm/490 nm and 460 nm/490 nm. Taking the colony laser fluorescence microscope as a platform, and using a laser beam ex

关 键 词：农产品 荧光 带衣花生米 黄曲霉素 分选 

分 类 号：TS201.3[轻工技术与工程—食品科学]