基于高通量测序技术的阳极生物膜群落结构  

Community Structure of Anode Biofilm by High-Throughput Sequencing Technology

作　　者：王有昭[1] 朱彤[1] 谢元华[1] 李现瑾 

机构地区：[1]东北大学机械工程与自动化学院,辽宁沈阳110819

出　　处：《东北大学学报（自然科学版）》2016年第1期105-108,122,共5页Journal of Northeastern University(Natural Science)

基　　金：中国博士后科学基金资助项目(2015M570255);中央高校基本科研业务费专项资金资助项目(N140303002);辽宁省教育厅科学技术研究项目(L20150178);东北大学博士后科研基金资助项目(20150304)

摘　　要：利用高通量测序技术对阳极生物膜的微生物群落结构进行了分析.实验结果表明,高通量测序能够高效地获取电化学生物膜的群落结构信息,产电模式菌Geobacter属在生物膜中占据着优势地位,其数量比例达到30%左右,其次的主要菌属为Thermovirga,Thauera和Syntrophorhabdus.对比电化学活性丧失前后的生物膜发现,虽然Geobacter菌属在丧失了电化学活性的生物膜中仍然占据统治地位,但是放线菌门的细菌增长显著,其数量比例远高于正常阳极生物膜的0.5%,有可能是抑制生物膜电化学活性的因素之一.Microbial community structure of anode biofilm was analyzed using high-throughput sequencing technology. The results show that the technology can obtain community structure information from electrochemistry biofilm efficiently, and the exoelectrogentic modal bacteria Geobacter category is predominant in biological membrane, its quantity ratio reaches 30%; Thermovirga, Thauera and Syntrophorhabdus are also found in anode biofilm. However, through comparison of biofilm before and after electrochemical activity was lost, Geobacter still dominated in biofilm after the biofilm lost electrochemical activity, while Actinomycetes＇ proportion increased significantly, its quantity was much higher than that of the normal 0. 5%. So Actinomycetes may be one of the factors which inhibit the electrochemical activity of the biofilm.

关 键 词：阳极生物膜 生物电化学系统 高通量测序 微生物多样性 16S RRNA 

分 类 号：Q89[生物学]