湖泊沉积物微生物多样性研究方法的新进展  

New Progress of Research Methods for Microbial Diversity in Lake Sediment

作　　者：李国强[1,2] 薛林贵[1,2] 莫天录 杨蕊琪 李文伟[1,2] 

机构地区：[1]兰州交通大学化学与生物工程学院,甘肃兰州730070 [2]甘肃省极端环境微生物资源与工程重点实验室,甘肃兰州730070

出　　处：《兰州交通大学学报》2015年第6期12-16,37,共6页Journal of Lanzhou Jiaotong University

基　　金：国家自然科学基金(31260135);甘肃省科技支撑计划项目资助(1504FKCA015);教育部长江学者和创新团队发展计划项目资助(IRT0966)

摘　　要：湖泊沉积物在湖泊生态系统中扮演了一个非常重要的角色,它是微生物与湖泊生态环境的营养循环与物质交换的基础,因此,研究湖泊沉积物微生物多样性具有非常重要的意义.通过对近几年湖泊沉积物微生物多样性的研究方法进行综述,介绍了各种方法的原理及其应用特点等.最后指出,对于湖泊沉积物微生物多样性的研究应该以分子生物学方法为主体,同时将传统方法与分子生物学方法相结合,才能得到更为准确的微生物多样性信息.Lake sediment plays a very important role in the lake ecosystem. It is the basis of the nutrient cycle and the material exchange between the microbes and the lake ecological environ- ment. Therefore,it is great significant to study the microbial diversity in lake sediments. A series of studying methods have summarized for the microbial diversity in lake sediment microbes in re- cent years, and the principle and the applied features of these methods are introduced, etc. Final- ly, it is pointed out that the molecular biology method should be the main method to study the mi- crobial diversity, and the combination of the traditional method and molecular biology method can get more accurate information of microbial diversity.

关 键 词：湖泊沉积物 微生物多样性 PCR 测序技术 

分 类 号：X172[环境科学与工程—环境科学]