根际不同供氮水平对紫青菜生长和营养品质的影响  

Effect of Nitrogen Supply Level in Rhizosphere on Growth and Nutritional Quality of Purple Pak-choi

作　　者：刘爱荣[1] 张远兵[2] 黄守程[1] 胡能兵[3] 林平[3] 

机构地区：[1]安徽科技学院生命科学学院,安徽凤阳233100 [2]安徽科技学院建筑学院,安徽凤阳233100 [3]安徽科技学院农学院,安徽凤阳233100

出　　处：《热带亚热带植物学报》2016年第1期56-62,共7页Journal of Tropical and Subtropical Botany

基　　金：安徽省科技厅攻关计划项目(1301031030);安徽省科技厅农业科技成果转化项目(1404032007)资助~~

摘　　要：为了解根际供氮对紫青菜(Brassica campestris ssp.chinensis var.communis)生长和营养品质的影响,用不同水平的NH_4NO_3处理,对紫青菜的生理特性和营养品质进行研究。结果表明,随着供氮水平的增加,紫青菜的鲜质量、干质量、根系活力和游离氨基酸含量均呈先上升后略下降趋势;硝酸还原酶(NR)活性、叶绿素含量、NO_3~–含量和可溶性蛋白质含量均呈上升趋势;DPPH·自由基清除率、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)同工酶和过氧化氢酶(CAT)同工酶活性等均提高;而花青素苷相对含量、可溶性糖含量、维生素C(Vc)含量、总酚含量和FRAP值均呈下降趋势。根际NH_4NO_3为75~300 mg L^(–1)时,紫青菜的鲜质量和干质量较低;NH_4NO_3达1200 mg L^(–1)时,其鲜质量和干质量又低于600 mg L^(–1)处理的,植株积累较多NO_3~–,导致营养品质下降,同时根际也积累NH4NO3。因此,600 mg L^(–1)的根际NH_4NO_3是适宜的氮水平,其鲜质量和干质量均最高、营养均衡、抗氧化能力强。In order to understand the effects of rhizosphere N supply on the growth and nutritional quality of purple pak-choi(Brassica campestris ssp. chinensis var. communis), the physiological and nutrition characters were studied treated with different levels of NH_4NO_3. The results showed that the fresh and dry weight, root activity, free amino acid content of purple pak-choi increased initially and then slightly decreased with the N level increment. Nitrate reductase(NR) activity, contents of chlorophyll, NO_3~– and soluble protein, and DPPH· radical scavenging rate increased, activities of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) isozymes showed upward trend, while the contents of relative anthocyanin, soluble sugar, vitamin C(Vc) and total phenols, and FRAP value decreased. Meantime, the fresh and dry weight of purple pak-choi treated with 75-300 mg L^(–1) NH_4NO_3 was low. The fresh and dry weight treated by 1200 mg L^(–1) NH_4NO_3 was lower than that by 600 mg L^(–1) NH_4NO_3, more NO_3~– accumulated in plant led to decline of nutrient quality, and NH_4NO_3 deposited in rhizosphere. So, the optimum level of NH_4NO_3 was 600 mg L^(–1) because of high fresh and dry weight, balanced nutrition and strong antioxidant ability.

关 键 词：氮素 紫青菜 生长 营养品质 抗氧化能力 

分 类 号：S634[农业科学—蔬菜学]