傣药人面果根部清除DPPH自由基能力及实验方法比较  

Comparative study of DPPH radical scavenging efficiency of methanol extracts from Garcinia xanthoymus based on two methods

作　　者：杨红梅[1] 王忠诚[2] 余抒寰 杜刚[2] 杨海英[1] 

机构地区：[1]云南民族大学化学与环境学院,云南昆明650500 [2]云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2016年第1期1-4,共4页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(21462051);云南民族大学民族药资源化学国家民委-教育部重点实验室开放基金(MZY1401)

摘　　要：分别采用高效液相色谱法和可见分光光度法检测傣药人面果根部甲醇提取物清除DPPH自由基的能力.高效液相色谱法测得人面果根部甲醇提取物清除DPPH自由基的IC50为0.116 mg/m L,可见分光光度法测得人面果根部甲醇提取物清除DPPH自由基的IC50为0.108 mg/m L,且DPPH自由基清除率与人面果根部甲醇提取物质量浓度呈正相关.统计学分析结果表明,2种实验方法的实验结果之间无显著性差异,2种方法均可用于傣药人面果对DPPH自由基清除能力的研究.The DPPH radical scavenging activities of methanol extracts of Garcinia xanthoymus were determined by using HPLC and visible spectroscopy. The results showed some positive correlation between DPPH radical scaven- ging rate and the concentration of methanol extracts of Garcinia xanthoymus. Its IC_50 values measured by HPLC method was 0. 116 mg/mL,and the corresponding vales measured by the visible spectroscopy was 0. 108 mg/mL. In addition, the statistical analysis indicated that the results of the two methods were not significantly different. It concludes that both methods could be used for the analysis of DPPH radical scavenging activities of the methanol ex-tracts of Garcinia xanthoymus.

关 键 词：人面果 DPPH自由基 高效液相色谱法 可见分光光度法 

分 类 号：R285.5[医药卫生—中药学]