油棕仁贮藏蛋白质亚基组成和含量分析  

Study on Subunits of Oil Palm Kernel Storage Proteins

作　　者：张璐[1,2] 郑亚军[1,3] 李艳[1] 张有林[1] 张润光[1] 张玉峰[3] 

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710119 [2]海南大学园艺园林学院,海南海口570228 [3]中国热带农业科学院椰子研究所,海南文昌571339

出　　处：《安徽农业科学》2016年第1期8-11,共4页Journal of Anhui Agricultural Sciences

基　　金：国家科技支撑项目(2012BAD31B03);海南省重大科技项目(ZDZX2013011)

摘　　要：［目的］为油棕仁中蛋白质的进一步研究与开发利用提供指导。［方法］ 采用非变性聚丙稀酰胺凝胶电泳（NativePAGE）和十二烷基硫酸钠－聚丙稀酰胺凝胶电泳（SDSPAGE）对油棕仁中贮藏蛋白质亚基进行研究，分析油棕仁蛋白主要亚基的组成、分子量、含量、二硫键的位置及种间差异。［结果］ 油棕仁中贮藏蛋白质在浓缩胶浓度为7.5%、分离胶浓度为12%的SDSPAGE凝胶系统中可以得到很好的分离。在油棕仁贮藏蛋白中含有6条主要亚基，分子量范围为18.8 ~51.5 kDa，主要是中、小分子量亚基；各亚基之间含一定量的二硫键。非变性凝胶电泳分析表明，油棕仁贮藏蛋白中含有4个不同分子量、不同电荷的组分。［结论］ 该研究所选的不同油棕品种之间亚基的组成和含量没有显著性差异。[ Objective ] The research aimed to provide the guidance for the further study and the development and utilization of protein in oil palm kernel. [ Method] The subunits of storage proteins in oil palm kernel were studied by SDS-PAGE and Native-PAGE, including their content, molecular weight, content of disulfide bonds and variance difference. [ Result] Storage proteins in oil palm kernel were well separated by SDS-PAGE with 12% separation gel and 7.5 % pile gel. Under this condition, the storage proteins in oil palm kernel were separated into 6 main subunits, with a molecular weight range of 18.8 ~ 51.5 kDa. This meant that the main subunits were lower molecular weight in oil palm kernel storage proteins. Compared with the result of SDS-PAGE with Native-PAGE, there were some disulfide bonds among the subunits of oil palm ker- nel storage proteins. There were four peptides with different molecular and different electric charge separated by Native-PAGE. [ Conclusion] There was no significant difference between the two kinds of oil palm.

关 键 词：油棕仁 贮藏蛋白 亚基 SDSPAGE 

分 类 号：S789.5[农业科学—林学] TS201[轻工技术与工程—食品科学]