儿茶素对胃癌细胞SGC-7901和MGC-803迁移及侵袭的影响  

Effects of catechin on invasion and metastasis in gastric carcinoma cells SGC-7901 and MGC-803

作　　者：杜培培[1] 刘宗文[2] 张艳[1] 张钰浩[3] 尚淑芳[1] 贾丹辉[1] 

机构地区：[1]郑州大学基础医学院药理学教研室,郑州450001 [2]郑州大学第二附属医院肿瘤放疗科,郑州450014 [3]郑州大学临床医学系,郑州450052

出　　处：《郑州大学学报（医学版）》2016年第1期51-55,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省高等学校重点科研项目基础研究计划基金资助项目15A310031;郑州市科技创新团队基金资助项目131PCXTD628

摘　　要：目的:探讨儿茶素是否作用于酸敏感离子通道(ASICs),进一步研究儿茶素是否通过ASICs对胃癌细胞迁移和侵袭产生影响。方法:采用MTT法检测儿茶素(0、50、100、200、400μmol/L)对胃癌细胞SGC-7901和MGC-803增殖的影响。将细胞分为对照组,100μmol/L阿米洛利组,50、100、200μmol/L儿茶素组,采用免疫细胞化学方法检测ASIC1的表达情况,Transwell实验和划痕实验检测细胞的迁移和侵袭情况。采用全细胞膜片钳技术观察对照组、100μmol/L阿米洛利组、400μmol/L儿茶素组细胞的电流情况。结果:ASIC1在各组细胞中均有表达,并且与SGC-7901细胞相比,MGC-803细胞中ASIC1的表达较低(P<0.05);与对照组相比,儿茶素可以抑制ASIC1的表达,并且儿茶素的抑制作用弱于阿米洛利的抑制作用(P均<0.05)。与SGC-7901细胞相比,MGC-803细胞的侵袭和迁移能力较弱(P均<0.05);与对照组相比,儿茶素可减弱2种胃癌细胞的侵袭能力和迁移能力,并且儿茶素的抑制作用弱于阿米洛利的抑制作用(P均<0.05)。MGC-803细胞的ASICs电流小于SGC-7901细胞的ASICs电流(P<0.05);与对照组相比,儿茶素可使ASICs电流减弱,并且儿茶素的抑制作用弱于阿米洛利的抑制作用(P均<0.05)。结论:儿茶素可以通过抑制ASICs的表达抑制胃癌细胞的迁移和侵袭。Aim： To investigate the effects of catechin on ASICs,and explore the effects of catechin on invasion and metastasis of gastric carcinoma cells. Methods： The SGC-7901 and MGC-803 cells were treated with 0,50,100,200,and 400μmol /L catechin and the proliferation of the cells were observed by MTT. The SGC-7901 and MGC-803 cells were allocated into control group,100 μmol / L amiloride group,50,100,200 μmol / L catechin groups; the expression of ASIC1 protein was detected by immunocytochemistry,and the changes of cell invasion and metastasis were detected by wound-healing migration assay and Transwell migration assay,respectively. Finally,the ASICs current was analyzed by the whole cell patch clamp.Results： Expression of ASIC1 protein in MGC-803 cells was lower that in SGC-7901 cells（ P〈0. 05）. Compared with the control group,catechin reduced ASIC1 expression,and the inhibitive effect of catechin on ASIC1 expression was weaker than that of Amiloride（ P〈0. 05）. The invasion and metastasis ability of MGC-803 cells were weaker than that of SGC-7901 cells（ P〈0. 05）. Compared with the control group,catechin could reduce invasion and metastasis ability of MGC-803 and SGC-7901 cells,and the inhibitive effect of catechin was weaker than that of Amiloride（ P〈0. 05）. The ASICs current of MGC-803 was lower than that of SGC-7901 cells（ P〈0. 05）. Compared with the control group,catechin could reduce ASICs current of MGC-803 and SGC-7901 cells,and the inhibitive effect of catechin was weaker than that of Amiloride（ P〈0. 05）. Conclusion： Catechin could inhibit invasion and metastasis ability of gastric carcinoma cells by reducing the expression of ASICs.

关 键 词：胃癌 酸敏感离子通道 阿米洛利 儿茶素 

分 类 号：R735.2[医药卫生—肿瘤]