大蒜辣素对高糖环境下肝癌细胞HepG2/mdr耐药性影响及其机制研究  

Mechanism of allicin on drug resistance of HepG2 / mdr hepatocellular carcinoma cells in high glucose

作　　者：燕丹[1] 童本定[1] 丁年羊[1] 吴楠[1] 王蕾[1] 唐文婧[1] 魏青[1] 

机构地区：[1]江苏省肿瘤医院,江苏南京210009

出　　处：《东南大学学报（医学版）》2015年第6期987-991,共5页Journal of Southeast University(Medical Science Edition)

摘　　要：目的:探讨大蒜辣素(allicin)对高糖环境下肝癌细胞HepG2/mdr耐药性影响及其机制。方法:采用细胞计数试剂盒(Cell Counting Kit-8)检测细胞株HepG2/mdr活性;RT-PCR检测allicin作用后HepG2/mdr中晚期糖基化终产物受体(receptor for advanced glycation endproducts,RAGE)、核转录因子-κB(nuclear factor-kappa B,NF-κB)、多药耐药基因-1(multidrug resistance-1,MDR1)的转录水平;蛋白质免疫印迹法(Western blotting)检测allicin作用后HepG2/mdr细胞中相关基因的蛋白表达。结果:HepG2/mdr细胞呈对数生长,可以作为后续实验的靶细胞。RT-PCR和Western blotting检测表明,随着allicin质量浓度的增加,RAGE、NF-κB、MDR1转录水平降低,蛋白表达量减少,呈现剂量依赖性。结论:allicin能通过影响肿瘤细胞NF-κB的核转录因子活性调控下游MDR1基因的表达,从而逆转高糖状态下HepG2/mdr细胞的耐药性。Objective： To investigated mechanism of allicin on drug resistance of HepG2 /mdr hepatocellular carcinoma cells in high glucose. Methods： Cell counting kit-8（ CCK-8） assay was used to assess the HepG2 / mdr cells activity,The expression change of mRNA and protein among RAGE、NF-κB and MDR1 were examined by RTPCR and Western-blot. Results： The results of CCK-8 assay showed that HepG2 / mdr cells present logarithmic growth could be used as target cells in the following study,RT-PCR and Western blotting experimental results showed that allicin could reduce transcription level and decrease protein expression of RAGE、NF-κB、MDR1. the phenomena were more obvious when allicin concentration increasing. Conclusion： Allicin could reverse drug resistance of tumor cells to therapy. It showed that MDR was dramatically down-regulated in a Allicin-dependent manner in response to NF-κB changes.

关 键 词：大蒜辣素 肝癌细胞HepG2/mdr 多药耐药 

分 类 号：R735.7[医药卫生—肿瘤]