响应面法优化鹿角菜中岩藻多糖的超声波辅助提取工艺  

Optimization of ultrasonic assisted extraction of fucoidan from Pelvetia siliquosa Tsenget C.F. Chang and evaluation of its antioxidant activities

作　　者：宋海燕[1] 何文辉[1] 张泽华[1] 袁荣荣[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《食品与发酵工业》2015年第12期212-218,共7页Food and Fermentation Industries

基　　金：国家水体污染控制与治理科技重大专项(No.2014ZX07101-012);上海高校水产学一流学科建设项目资助

摘　　要：采用超声波辅助提取鹿角菜中岩藻多糖,以岩藻多糖提取率为指标,研究超声时间、液料比、水浴温度和水浴时间4个参数对提取效果的影响,并采用Box-Benhnken设计(BBD)来优化提取工艺,确定提取条件为超声时间60 min,液料比51 m L∶g,水浴温度91℃,水浴时间5 h。在此条件下,岩藻多糖提取率达到5.961%,与理想预测值(5.993%)相比,相对误差小于5%;与传统水提法相比,提取总时间缩短了2.4 h,多糖提取率提高了0.79%。通过自由基体系评定发现,岩藻多糖具有较强的抗氧化活性,且还原力随着质量浓度的增加不断提高,对羟自由基(·OH)、超氧阴离子自由基(O^-_2·)和1,1-二苯基-2-三硝基苯肼(DPPH)自由基的半数抑制浓度IC_(50)分别为0.346、0.893和0.128 mg/m L;但与Vc相比,对羟自由基、超氧阴离子自由基清除作用较弱,但对DPPH的半数抑制浓度IC50小于Vc。Using ultrasonic assisted extraction of fucoidan from Pelvetia siliquosa Tsenget C. F. Chang（ P. siliquosa Tsenget C. F. Chang）,the effects of ultrasonic time,liquid-solid ratio,water bath temperature and water bath time on the extraction efficiency were studied. The optimum extraction conditions optimized by box benhnken design（ BBD） were ultrasonic time 60 min,liquid-solid ratio 51 m L/g,water bath temperature 91 ℃ and water bath time 5h. Under these conditions,the fucoidin extraction rate reached to 5. 961%,compared with an ideal predictive value（ 5. 993%）,the relative error was less than 5%; Compared with the traditional water extraction,the total time of extraction was shortened by 2. 4 h,polysaccharide extraction improved 0. 79%. Radical system assessment found that fucoidan has strong antioxidant activity,and also reducing power continuous improvement with the mass concentration increasing. IC50 of hydroxyl radical,superoxide anion radical and DPPH were 0. 346 mg / m L,0. 893 mg / m L and0. 128 mg / m L. Compared with VC,the scavenging effect of fucoidan on hydroxyl radical and superoxide anion radical was weak,but the IC50 of DPPH was less than VC. Therefore,ultrasonic assisted extraction proceed of fucoidan optimized by the response surface methodology was convenient and feasible. Fucoidan from P. siliquosa Tsenget C. F.Chang has strong antioxidant activity.

关 键 词：鹿角菜 岩藻多糖 超声波 提取 响应面 抗氧化活性 

分 类 号：TQ464.1[化学工程—制药化工]