分析化学综合新实验的设计研究  

Study of new comprehensive experiment design in analytic chemistry

作　　者：徐彦芹[1] 卞欢[1] 陈家状 刘浩[1] 

机构地区：[1]重庆大学化学化工学院,重庆400044

出　　处：《实验室科学》2015年第6期7-9,13,共4页Laboratory Science

基　　金：重庆市高等教育教学改革研究项目(项目编号:143055);第六届"重庆大学大学生科研训练计划"项目(项目编号:CQU-SRTP-2014377)

摘　　要：本实验以中药材金银花为样本,拟采用超声提取分离技术对其中的有效活性成分绿原酸进行提取,采用薄层层析法(TLC)对分离获得的绿原酸进行定性识别,进而采用高效液相色谱方法 (HPLC)对提取的绿原酸进行定量测试。该实验使学生对如何采用分析化学实验的分离和测试方法实现对中药材的质量控制的途径和方法形成一个全面的认识,适用于药学、制药工程等专业本科分析化学实验教学。In the experiment,the traditional Chinese medicines-lonicera japonica will be taken as the specimen. Chlorogenic acid,the effective active ingredients of it,can be extracted by ultrasonic ethanol extraction. The qualitative identification and quantitative analysis of chlorogenic acid will be conducted by TLC and by HPLC. By this experiment,students will form a more comprehensive understanding of the ways and methods of Chinese medicine quality control by using the techniques of separation and testing in analytic chemistry. This experiment is suitable for undergraduate experimental teaching of analytic chemistry for pharmacy specialty and pharmaceutical engineering specialty,etc.

关 键 词：实验教学 分析化学 新实验设计 金银花 绿原酸 

分 类 号：R284.2[医药卫生—中药学]