阿尔茨海默病患者ApoE等位基因频率与脂类代谢的相关性  

The relationship of apolipoprotein E allelic frequency and serum lipid levels in patients with Alzheimer′ s disease

作　　者：张婷[1] 胡国艳[2] 林裕龙[2] 

机构地区：[1]南方医科大学珠江医院检验医学部,广州市510282 [2]广州市脑科医院检验科,510370

出　　处：《实用医学杂志》2016年第1期127-129,共3页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2014A020212375)

摘　　要：目的:探讨阿尔茨海默病(AD)患者载脂蛋白E(ApoE)等位基因频率及与脂质水平的相关性。方法:采用DNA微阵列技术检测200例AD患者(AD组)及159例非AD健康老年人(对照组)的ApoE基因型,同时检测所有受检者的脂质水平,分析ApoE等位基因频率与脂质水平的相关性。结果:AD组ApoEε4等位基因频率(25.5%)高于对照组(7.9%)(P<0.05);人群中ApoE等位基因所对应的ApoE水平为ε2>ε3>ε4,差异有显著性(P<0.05);相同ApoE基因型的AD患者与健康人群间ApoE水平差异无显著性(P>0.05)。结论:ApoEε4等位基因频率与ApoE水平呈负相关,相同基因型的ApoE水平差异无显著性,提示AD患者ApoE总体水平低于健康人群的主要原因是AD患者中ApoEε4等位基因频率显著高于健康人群所致。Objective To investigate the relationship of apolipoprotein E（ApoE） allelic frequency and serum lipid levels in patients with Alzheimer＇s disease（AD）.Methods DNA microarray was used to detect the ApoE genotypes of AD patients（n = 200） and age-matched non-demented elderly control subjects（n = 159）.Serum lipid levels was measured by Immunoturbidimetric assay at the same time.We analyzed the ApoE genotype distribution and the relationship of apolipoprotein E（ApoE） allelic frequency and serum lipid levels.Results The ApoE ε4 allelic frequencies（25.5%） in AD group is higher than that of the control group（7.9%）（P〈0.05）.The ε2 allele was associated with a higher ApoE concentration,whereas with a mediate concentration in ε3 and the lowest concentration（P〈0.05） in ε4.Serum concentrations of ApoE showed no significant difference between AD patients and the healthy population who were with the same genotype（P〉0.05）.Conclusion The ApoE levels are negatively related to ApoE ε4 allele frequency and have no significant differences with the same genotype in AD and the control group,which suggests that lower serum ApoE levels in AD patients is caused by higher ApoE ε4 allelic frequency in AD than in healthy population.

关 键 词：阿尔茨海默病 载脂蛋白E 等位基因 DNA微阵列技术 

分 类 号：R749.16[医药卫生—神经病学与精神病学]