油茶籽饼中茶皂素定量检测方法研究  

Quantitative analysis of tea saponin from seed cake

作　　者：张团结[1] 熊道陵[1] 许光辉[1] 陈超[1] 陈金洲 

机构地区：[1]江西理工大学冶金与化学工程学院,江西赣州341000

出　　处：《食品工业科技》2016年第4期53-56,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(51364014);江西省科技厅资助项目(2013BBG70003);江西省级大学生创新创业训练计划项目(201310407037;201310407057;201410407030);江西省高校专业综合改革试点项目(SZG-14-01-01)资助

摘　　要：本文建立了一种快速、简单、准确测定油茶籽饼中茶皂素含量的方法。结果表明:采用分光光度法和高效液相色谱法对同含量的茶皂素测定结果较为吻合,相比之下分光光度法更为简单快捷。分光光度法是以香草醛-高氯酸作为显色剂,通过茶皂素甙元显色,于554 nm为检测波长。在样品待测液中,加入香草醛-高氯酸1.0 m L,在温度为60℃的条件下反应15 min,显色效果最佳。该方法精密度高,显色后在40 min内吸光度稳定,平均加标回收率为98.28%(RSD=3.86%)。本实验建立了分光光度法测定茶皂素含量的方法,具有良好的准确度、精密度和稳定性,可用于茶皂素含量的测定。In order to establish a rapid,simple and accurate method of determining the content of tea saponin,spectrophotometry and HPLC methods were used. The quantitative analysis results for the same content of tea saponins from the two improved analysis methods were very accordant and the former was more simple. The employed coloring reagents was vanillin-acetic acid and the color determination of saponin content based on aglycone at the wavelength of 554 nm. Optimal color condition for sample solution were obtained as follows：color reagents 1.0 m L,temperature 60 ℃,reaction time 15 min. The color of the samples were stabled within40 min and the average recovery was 98.28%（RSD =3.86%） with high precision. The method based on spectrophotometry including high accurate,precision and stability,it could be used for quantitative determination of tea saponin.

关 键 词：分光光度法 高效液相色谱法 茶皂素 甙元 

分 类 号：TS201.1[轻工技术与工程—食品科学]