李斯特菌免疫检测用胶体金的制备及抗体标记  

Preparation of colloidal gold and colloidal gold-labeled antibody used in immunoassay for detection of Listeria

作　　者：刘珊娜[1,2] 葛怀娜 孟繁桐 范志华[1,2] 刘金福[1,2] 

机构地区：[1]天津农学院食品科学与生物工程学院,天津300384 [2]天津市农副产品深加工技术工程中心,天津300384 [3]天津市德恒科技有限公司,天津300384

出　　处：《食品科技》2016年第1期321-324,共4页Food Science and Technology

基　　金：天津市科技支撑计划项目(14ZCZDNC00003)

摘　　要：单增李斯特菌是重要的食源性致病菌,可危害人体健康,造成经济损失。加强食品中单增李斯特菌的检测,是降低食品安全风险的有效手段。胶体金免疫层析技术以胶体金作为示踪物,结合抗原抗体反应应用于食品安全快速检测领域。通过柠檬酸三钠还原氯金酸的方法制备胶体金溶液。胶体金最佳制备条件为:25 m L 0.01%氯金酸水溶液中加入1 m L 1%柠檬酸三钠水溶液,反应时间5 min。利用李斯特菌菌体蛋白抗体标记胶体金来制备探针,最佳标记p H值为8.4,最佳标记蛋白抗体量为12μg/m L。制备的胶体金探针具有生物活性,可用于食品中单增李斯特菌快速检测方法的开发。Listeria monocytogenes is an important genus of food-borne pathogen which may damage human health and cause economic loss. Enhancement in detection of L. monocytogenes in food was an effective way of reducing the risk in food safety. Colloidal gold immunochromatography technology was based on colloidal gold as tracer and antigen-antibody reaction for rapid detection in food safety. The colloidal gold solution was prepared by citrate sodium reduction. The optimized condition for preparation of colloidal gold is addition of 1 m L 1% sodium citrate to 25 m L 0.01% HAu Cl4 and reaction for 5 min. AntiListeria antibody was conjugated to colloidal gold with optimized p H at 8.4 and optimized antibody amount of 12 μg/m L. The prepared colloidal gold-labeled antibody was bioactive, which was able to be used for developing rapid detection method for L. monocytogenes in food.

关 键 词：胶体金 柠檬酸三钠 标记 李斯特菌菌体蛋白抗体 

分 类 号：TS201.3[轻工技术与工程—食品科学]