高效液相色谱-串联质谱法检测茶叶中的赭曲霉毒素A  

Determination of ochratoxin A in tea by high performance liquid chromatography-tandem mass spectrometry

作　　者：莫瑾[1] 龚强[1] 周慧平[1] 白雪[1] 谭建锡[1] 彭梓[1] 黄迎波[1] 

机构地区：[1]湖南出入境检验检疫局检验检疫技术中心,国家食品安全检测重点实验室,食品安全科学技术湖南省重点实验室,长沙410004

出　　处：《食品安全质量检测学报》2016年第1期182-187,共6页Journal of Food Safety and Quality

基　　金：国家质检总局项目(2010IK133)~~

摘　　要：目的建立高效液相色谱-串联质谱联用技术检测茶叶中赭曲霉毒素A（OTA）的方法。方法用甲醇-2%Na HCO3溶液（60：40,V：V）提取样品中的赭曲霉毒素A,采用免疫亲和柱对茶叶中的赭曲霉毒素A净化,使用甲醇-5 mmol/L乙酸铵（含0.1%乙酸）为流动相,检测赭曲霉毒素A,采用正离子模式。结果本方法中赭曲霉毒素A在0.5~10 ng/m L质量浓度范围内具有良好的线性关系（r=0.996）,回收率在83.6%~92.5%之间,相对标准偏差在6.5%~9.1%之间,检出限为0.1μg/kg。结论该方法准确性好、灵敏度高,适用于茶叶样品的检测。Objective To establish a method for the determination of ochratoxin A （OTA） in tea by high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）. Methods Samples were extracted by methanol-2% sodium bicarbonate solution （60：40, V：V）, followed by clean-up on OchraTest affinity cartridge using methanol-5 mmol/L ammonium acetate solution in the presence of 0.1% formic acid as the mobile phase. OTA was detected in positive ion mode. Results A good linearity （r=0.996） was achieved for the target compounds in the range of 0.5-10 ng/mL. The recoveries of ochratoxin A in 4 tea samples were varied from 83.6% to 92.5% （n = 5） with the relative standard deviations from 6.5% to 9.1%. The limit of detection was 0.1μg/kg. Conclusion The method is accurate and sensitive, and suitable for the determination of ochratoxin A in tea samples.

关 键 词：高效液相色谱-串联质谱法 赭曲霉毒素A 免疫亲和柱 茶叶 

分 类 号：O657.63[理学—分析化学] TS272.7[理学—化学]