Jinmaitong(筋脉通)Alleviates the Diabetic Peripheral Neuropathy by Inducing Autophagy  

作　　者：屈岭 张宏 顾蓓 戴威 吴群励 孙连庆 赵丽 石玥 梁晓春 

机构地区：[1]Department of Traditional Chinese Medicine,Peking Union Medical College Hospital,Peking Union Medical College,Chinese Academy of Medical Sciences [2]Cell Center,Institute of Basic Medical Science,Peking Union Medical College,Chinese Academy of Medical Sciences [3]Department of Electron Microscope,Institute of Basic Medical Science,Peking Union Medical College,Chinese Academy of Medical Sciences

出　　处：《Chinese Journal of Integrative Medicine》2016年第3期185-192,共8页中国结合医学杂志（英文版）

基　　金：Supported by the Natural Science Foundation of China(No.30572438);the Beijing Science Nuture Foundation(No.7132189);Science Foundation of Peking Union Medical College Hospital(No.2013-098);Youth Foundation of Peking Union Medical College Hospital

摘　　要：Objective： To observe the deregulation of autophagy in diabetic peripheral neuropathy （DPN） and investigate whether Jinmaitong （筋脉通, JMT） alleviates DPN by inducing autophagy. Methods： DPN models were established by streptozotocin-induced diabetic rats and Schwann cells （SCs） cultured in high glucose medium. The pathological morphology was observed by the improved Bielschowsky＇s nerve fiber axonal staining and the Luxol fast blue-neutral red myelin staining. The ultrastructure was observed by the transmission electron microscopy. Beclinl level was detected by immunohistochemistry and Western blot. The proliferation of cultured SCs was detected by methylthiazolyldiphenyl-tetrazolium bromide. Results： Diabetic peripheral nerve tissues demonstrated pathological morphology and reduced autophagic structure, accompanied with down-regulation of Beclinl. JMT apparently alleviated the pathological morphology change and increased the autophagy [in vivo, Beclinl integral optical density （IOD） value of the control group 86.6 ± 17.7, DM 43.9± 8.8, JMT 73.3 ± 17.8, P〈0.01 or P〈0.05, in vitro Beclinl IOD value of the glucose group 0.47 ± 0.25 vs the control group 0.88 ± 0.29, P〈0.05]. Consequently, inhibition of autophagy by 3-methyladenine resulted in a time- and concentration- dependent decrease of the proliferation of SCs （P〈0.05, P〈0.01）. Conclusions： Down-regulation of autophagy in SCs might contribute to the pathogenesis of DPN. JMT alleviates diabetic peripheral nerve injury at least in part by inducing autophagy.Objective： To observe the deregulation of autophagy in diabetic peripheral neuropathy （DPN） and investigate whether Jinmaitong （筋脉通, JMT） alleviates DPN by inducing autophagy. Methods： DPN models were established by streptozotocin-induced diabetic rats and Schwann cells （SCs） cultured in high glucose medium. The pathological morphology was observed by the improved Bielschowsky＇s nerve fiber axonal staining and the Luxol fast blue-neutral red myelin staining. The ultrastructure was observed by the transmission electron microscopy. Beclinl level was detected by immunohistochemistry and Western blot. The proliferation of cultured SCs was detected by methylthiazolyldiphenyl-tetrazolium bromide. Results： Diabetic peripheral nerve tissues demonstrated pathological morphology and reduced autophagic structure, accompanied with down-regulation of Beclinl. JMT apparently alleviated the pathological morphology change and increased the autophagy [in vivo, Beclinl integral optical density （IOD） value of the control group 86.6 ± 17.7, DM 43.9± 8.8, JMT 73.3 ± 17.8, P〈0.01 or P〈0.05, in vitro Beclinl IOD value of the glucose group 0.47 ± 0.25 vs the control group 0.88 ± 0.29, P〈0.05]. Consequently, inhibition of autophagy by 3-methyladenine resulted in a time- and concentration- dependent decrease of the proliferation of SCs （P〈0.05, P〈0.01）. Conclusions： Down-regulation of autophagy in SCs might contribute to the pathogenesis of DPN. JMT alleviates diabetic peripheral nerve injury at least in part by inducing autophagy.

关 键 词：diabetic peripheral neuropathy Schwann cells AUTOPHAGY BECLINL Jinmaitong Chinese medicine 

分 类 号：R285.5[医药卫生—中药学]